诱导子和前体协同添加对悬浮培养的鸡血藤细胞产异黄酮的影响  

Synergistic effects of elicitors and precursors on the synthesis of isoflavones in cell suspension culture of Spatholobus suberectus Dunn.

作　　者：吴桂容[1] 曲芬霞[1] 何彩梅[1] 陈春岚[1] 陈兴田[1] 

机构地区：[1]贺州学院化学与生物工程学院,广西贺州542800

出　　处：《广东农业科学》2016年第5期129-134,共6页Guangdong Agricultural Sciences

基　　金：广西自然科学基金(2012GXNSFBA053055)

摘　　要：在鸡血藤细胞悬浮培养的动力学基础上,研究诱导子(茉莉酸甲酯)、前体(苯丙氨酸、乙酸钠)以及两者协同添加对鸡血藤细胞产异黄酮的影响。结果表明:悬浮培养的鸡血藤细胞的异黄酮合成与细胞生长之间存在半偶联关系,指数生长期前期的第3d有最大生长加速度,此时是最优添加时间。诱导子和前体单独添加均能促进异黄酮的合成,茉莉酸甲酯、苯丙氨酸和乙酸钠的最佳添加浓度分别为100、300、100μmol/L,异黄酮含量分别为对照的1.34、1.83和1.57倍。诱导子和前体协同添加对异黄酮合成的促进作用强于单独添加,茉莉酸甲酯+苯丙氨酸+乙酸钠处理下异黄酮含量高达19.32mg/L,为对照的3.62倍。The study investigated the effects of elicitors （methyl jasmonate,MJ）,precursors （phenylalanine,Phe and sodium acetate,Na Ac） and their synergistic effects on the synthesis of isoflavones in cell suspension culture of Spatholobus suberectus Dunn.,based on the result of kinetics research. The results showed that a semi-coupling relationship existed between cell growth of S. suberectus and isoflavone synthesis. At the 3rd d,the maximum acceleration of growth was found,indicating that it＇s suitable adding time for elicitors and precursors could promote the isoflavone synthesis,as the best added concentration for MJ,Phe and Na Ac were 100 μmol/L,300 μmol/L and 100 μmol /L,which were 1.34,1.83 and 1.57 times to the control,respectively. The synergistic addition of elicitors and precursors significantly promoted isoflavone synthesis,with isoflavone content up to 19.32 mg/L in MJ＋Phe＋Na Ac treatment （as 3.62 times to the control）. This study provided the theory basis for scale amplification in suspension cell culture of S. suberectus.

关 键 词：鸡血藤 细胞悬浮培养 异黄酮 诱导子 前体 协同作用 

分 类 号：Q814[生物学—生物工程]