激素诱导去势后绵羊骨质疏松动物模型的椎体微观结构观察及力学性能研究  

The study of microstructure and mechanical properties of the lumbar vertebrae in glucocorticoid and ovariectomy-induced osteoporotic sheep

作　　者：崔轶[1] 李军[1] 陆声[1] 王毅[1] 何晓清[1] 徐永清[1] 

机构地区：[1]成都军区昆明总医院,昆明650032

出　　处：《中国骨质疏松杂志》2016年第7期818-823,836,共7页Chinese Journal of Osteoporosis

基　　金：全军后勤科研计划面上项目(CCD14J003)

摘　　要：目的运用去势+糖皮质激素的方法,建立绵羊骨质疏松椎体模型,并通过Micro-CT观察其微观结构变化与其生物力学之间的关系。方法采用完全随机的设计方法,选用3±0.5岁绵羊24只,随机分配至3个处理组:A组(去势组),B组(去势+激素组),C组(假手术组),每组8只绵羊。观察时间为经过相应处理后9,12月。在每个时间点,处死一定数量的绵羊,然后检测各组在各时间点上椎体的骨密度(BMD);生物力学性能变化:测试最大轴向拔出力(Fmax)和最大抗压强度及其能量吸收值.同时切取部分椎体行Micro-CT检查,对各组各个时间点绵羊腰椎骨小梁微观结构进行观察。结果随着时间的延长,A,B组较C组均出现BMD及生物力学性能下降,显微CT观察见骨小梁空间结构呈骨质疏松样变化,且在同一时间点B组较A组向骨质疏松样变化更显著。结论运用去势+糖皮质激素的方法,可快速的建立绵羊骨质疏松动物模型,且经激素诱导9个月及12个月的去势绵羊均是较为理想的骨质疏松动物模型。Obje ctive To establish osteoporotic lumbar vertebrae model in sheep using glucocorticoid injection and ovariectomy,and to observe the changes of microstructure and mechanical properties and to study the relationship. Me thods Twenty-four 3 ±0. 5 years old adult sheep were randomly selected and divided into 3 groups： Group A（ OVX sheep group）,Group B（ steroidtreated OVX sheep group）,and Group C（ sham group）,with 8 sheep in each group. Observing time was 9 and 12 months after the treatment. At each time point,several sheep in each group were killed. Bone mineral density（ BM D） of the lumbar vertebrae,biomechanics,and maximum axial pullout force and uniaxial compression were determined. Partial vertebrae were scanned using micro-CT for observing the microstructure of the cancellous bone on different time points. Re sults With the time extension,BM D and mechanic properties of sheep in group A and B decreased compared to those in group C. The microstructure of cancellous bone appeared osteoporotic changes in group B compared to that in group A at micro-CT. Conclusion Osteoporosis sheep model can be established effectively by the method of glucocorticoid injection and ovariectomy rapidly. In 9 months or 12 months the model is ideal.

关 键 词：骨质疏松 糖皮质激素 骨密度 生物力学 动物模型 绵羊 显微CT 

分 类 号：R332[医药卫生—人体生理学]