关于补肾中药防治骨关节炎分子机制的体外实验研究  

Study of the mechanism of benefiting kidney herbs in the prevention and treatment of osteoarthritis in vitro

作　　者：李佳[1] 朱爱松[1] 谢晚晴[1] 郑洪新[1] 

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中国骨质疏松杂志》2016年第7期877-882,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学青年基金项目(81403286);辽宁省重点实验室项目(LZ2015052)

摘　　要：目的观察补肾中药单体淫羊藿苷、补骨脂素、齐墩果酸、二苯乙烯苷对间充质细胞中BMP2、BMP4、RUNX2表达的影响,以探讨和阐释肾中精气对关节软骨的调控作用及其分子机制,以丰富、补充和发展"肾藏精主骨"脏象理论。方法取1月龄野生型小鼠双侧后肢骨,分离间充质细胞(P1),分为空白组、对照组、淫羊藿苷组、补骨脂素组、齐墩果酸组和二苯乙烯苷组,实时定量PCR法检测BMP2、BMP4、RUNX2基因表达;Western-Blotting法检测BMP2、BMP4、RUNX2蛋白表达含量。结果1与成骨对照组相比,四个中药组BMP4、RUNX2基因表达均显著增高(P<0.05),与成骨对照组相比,淫羊藿组和二苯乙烯苷组BMP2基因表达显著增高(P<0.05);2与成骨对照组比较,补四个中药组BMP2、BMP4蛋白表达均有不同程度增强;淫羊藿苷组和二苯乙烯苷组RUNX2蛋白表达较对照组增强。结论 1实验用四种补肾中药单体能够上调BMP2、BMP4、RUNX2基因和蛋白表达;2补肾中药对骨关节炎的防治作用可能与本研究的分子机制有关;3"肾主骨"理论包含了肾对关节软骨的调控作用。Obje ctive To evaluate the effect of icariin,psoralen,oleanolic acid,and TSG on the expression of BM P 2,BM P 4,and Runx2,and to explore the function and molecular mechanism of benefiting kidney herbs in regulation of articular cartilage for extending the conception of kidney governing bone theory in Chinese medicine. Me thods M esenchymal cells were collected from the bilateral lowextremities of 1-month old wild type mice and divided into blank control,control,icariin,psoralen,oleanolic acid,and TSG groups. The expressions of BM P2,BM P4,and RUNX2 genes were detected using real-time quantitative PCR. Western blotting was performed to examine the protein expression of BM P2,BM P4,and RUNX2. Re sults 1） Comparing with that in control group,gene expression of BM P4 and RUNX2 significantly increased in the 4 herb groups（ P〈0. 05）. BM P2 gene expression was higher in icariin group and TSG group（ P〈0. 05）. 2） Comparing with that in control group,protein expression of BM P2 and BM P4 increased in different extend. Runx2 protein expression increased in icariin group and TSG group. Conclusions1） Icariin,psoralen,oleanolic acid,and TSG up-regulate the gene and protein expression of BM P2,BM P4,and RUNX2. 2） The function of prevention and treatment of osteoarthritis with benefiting kidney herbs is associated with the molecular mechanism of this study. 3） The kidney governing bone theory involves the regulation of articular cartilage by the kidney.

关 键 词：中医中药 肾 关节软骨 淫羊藿苷 补骨脂素 齐墩果酸 二苯乙烯苷 

分 类 号：R68[医药卫生—骨科学]