丙肝患者血清抗-HCV和HCVRNA检测效果分析  

An Analysis of Anti-HCV and HCV RNA Detection in Sera of Patients with Hepatitis C

作　　者：陈冬 

机构地区：[1]陕西省西安市中心血站,710061

出　　处：《临床输血与检验》2016年第4期374-376,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的比较ELISA法检测抗-HCV和荧光PCR法检测HCV RNA的效果,分析荧光PCR法在血站开展的必要性,为临床输血安全提供可靠依据。方法对2013年5月1日~2014年4月31日采集的149 200份无偿献血者标本进行HCV的检测。结果 ELISA法检测抗-HCV阳性标本610例,阴性标本148590例,610例阳性标本中用荧光PCR检测出HCV RNA阳性490例,占80.32%,148 590份抗-HCV阴性标本中HCV RNA阳性298例,占0.2%。结论用ELISA法检测抗-HCV,去除抗-HCV阳性血液,在此基础上对抗-HCV阴性血液加做HCV RNA核酸检测可以确保输血安全。Objective ELISA detection for anti HCV was compared with fluorescent PCR detection for HCV RNA in blood bank so as to evaluate the necessity of the fluorescent PCR detection for the safety ensurance of clinical blood transfusion. Methods 149200 blood samples were collected to detect the hepatitis C virus from April 31, 2014 to May 1, 2013. Results ELISA detection showed 610 cases of positive anti- HCV specimens, and 148590 cases of negative specimens.. Among the 610 positive cases fluorescent PCR test gave rise to 490 cases of positive HCV-RNA（80.32%,49 / 610）;in 148 590 samples with negative anti-HCV 298 cases were identified for HCV-RNA by fluorescent PCR test.（0.2%,298 /148 590）. Conclusion Additional application of HCV RNA detection to the individuals who exhibited negative results of anti-HCV tested by ELISA for may ensure the safety of clinical blood transfusion.

关 键 词：丙型肝炎病毒 荧光-PCR HCV RNA 

分 类 号：R512.63[医药卫生—内科学] R446.11[医药卫生—临床医学]