天府肉鸭胸腺发育中Fas、FasL和P53蛋白表达的动态研究  

The Expression Patterns of Fas, FasL and P53 Protines During Thymus Development of the Tianfu Duck

作　　者：蒋敏[1] 方静[1] 陈正礼[1] 唐丽[1] 

机构地区：[1]四川农业大学动物医学院,成都611130

出　　处：《动物学杂志》2016年第4期599-605,共7页Chinese Journal of Zoology

基　　金：教育部长江学者创新团队发展计划项目(No.0848)

摘　　要：凋亡相关因子（Fas）、凋亡相关因子配体（FasL）和磷蛋白53（P53）是细胞发生凋亡的重要调控因素。本文研究了天府肉鸭（Anas platyrhynchos domestica）胸腺发育过程中Fas、FasL和P53蛋白的动态表达,旨在更深入地认识胸腺细胞自然凋亡的调控机制。将65羽天府肉鸭分为13组,即22、24、26天胚龄,胚后0（新生雏）、3、5、8、14、17、20、26、29、32周龄。分别采取各组鸭的胸腺组织,4%多聚甲醛固定,常规石蜡切片,Fas、Fas L及P53蛋白免疫组织化学染色,镜检并进行阳性细胞计量分析。结果显示,Fas阳性反应见于胸腺淋巴细胞,其阳性率在22～26天胚龄无明显变化;新生雏显著升高,随后趋于恒定,直到17周龄,20周龄以后呈上升趋势。Fas L阳性反应见于胸腺淋巴细胞及上皮细胞,在胚胎及胚后发育过程中,其淋巴细胞阳性率无明显变化。P53阳性反应见于胸腺皮质淋巴细胞和胸腺上皮细胞,其淋巴细胞阳性率在各组中无显著性差异。结果说明Fas,Fas L和P53蛋白在天府肉鸭胸腺发育中呈现出不同表达规律。Factor associated suicide（Fas）, Fas ligand（Fas L） and phosphorus protein 53（P53） are important modulators in cell apoptosis. The expression patterns of Fas, Fas L and P53 proteins in thymus during embryonic and post-embryonic development of Tianfu Duck（Anas platyrhynchos domestica） were studied in order to deeply understand the regulatory mechanism of natural apoptosis of thymocytes. A total of 65 ducks were divided into 13 groups as follows： 22, 24 and 26 days embryonic stage, and 0（neonatal stage）, 3, 5, 8, 14, 17, 20, 26, 29 and 32 weeks after hatching. Thymic tissue from each group was collected, and immediately fixed in 4% paraformaldehyde. After fixation for 24 h, tissues were dehydrated, paraffin-embedded, sectioned at 5 μm, and stained for immunohistochemistry. Sections were observed under light microscope and microscopic analyses were performed. Results showed that Fas protein was distributed in the thymic lymphocytes（Fig. 1a﹣d）. The Fas-positive lymphocyte ratios showed no significant changes during the embryonic stage（P 0.05）. However, the values increased at the neonatal stage（P 0.05） and kept stable until 17 weeks after hatching（P 0.05）, and then showed an increased tendency from 20 to 32 weeks（Table 1）. The Fas L protein was observed in the thymic lymphocytes and epithelial cells（Fig. 1e﹣g）. The Fas L positive lymphocyte ratios had no significant changes during the embryonic and post-embryonic development（P 0.05）（Table 1）. The P53 protein was expressed in the thymic lymphocytes of cortex and the epithelial cells（Fig. 1h﹣k）. The P53-positive lymphocyte ratios showed no significant variations among groups（P 0.05）（Table 1）. This study indicates that Fas, Fas L and P53 proteins show different expression patterns during the development of thymus in Tianfu Ducks.

关 键 词：天府肉鸭 胸腺 凋亡相关因子(Fas) 凋亡相关因子配体(FasL) 磷蛋白53(P53) 免疫组化 

分 类 号：S834.89[农业科学—畜牧学] S852[农业科学—畜牧兽医]