全反式维A酸对HaCaT细胞中皮肤屏障相关因子Flg及Klf4的影响  

The Effect of All-trans Retinoic Acid on Expression of Klf4,Flg in HaCaT Cell

作　　者：郭坤[1] 肖生祥[1] 耿松梅[1] 

机构地区：[1]西安交通大学第二附属医院皮肤病院,陕西西安710004

出　　处：《中国皮肤性病学杂志》2016年第8期776-778,共3页The Chinese Journal of Dermatovenereology

基　　金：教育部新世纪人才支持计划(NCET-10-0673);国家自然科学基金资助(No.81171490);资生堂第一届DQ基金

摘　　要：目的研究全反式维A酸作用于人角质形成细胞HaCaT细胞后,通过分子水平检测Flg及Klf4的变化,以明确维A酸对皮肤屏障相关因子的影响及可能分子机制。方法 1μmol/L的全反式维A酸作用HaCaT 36h后,检测Flg,Klf4在基因及蛋白水平的变化。结果全反式维A酸作用HaCaT细胞36h后,实时定量PCR法及免疫印迹技术结果显示实验组Flg,Klf4表达下降。结论全反式维A酸作用于HaCaT细胞后,引起Flg及Klf4在基因及蛋白水平表达的下调,从分子生物学角度提示全反式维A酸可引起皮肤屏障的破坏。Objective To investigate whether skin barrier related molecules Flg and Klf4 was affected by ATRA（all-transretinoic acid）in HaCaT cell.Methods Pre-treated the HaCaT cells with ATRA（1uM）for 36h were used to detect the expression of Flg and Klf4 by Western blot and Realtime-PCR assay.Results Comparing with the control group,the expression level of Flg,Klf4 was suppressed after treatment by ATRA for 36h.Conclusion The expression of Flg,Klf4 was down-regulated in gene and protein level Results In cultured HaCaT cells treated by ATRA（1μmol/L）for 36h,ATRA could cause the destruction of skin barrier in molecular biology.

关 键 词：角质形成细胞 维A酸 Flg KLF4 

分 类 号：R751[医药卫生—皮肤病学与性病学]