巴西橡胶树乳管细胞HblMYC3互作蛋白筛选与功能分析  

Screening and in vitro Functional Analysis of the Hbl MYC3 Interacted Proteins in Laticifer Cells of Hevea brasiliensis

作　　者：王靖[1,2] 邓小敏[2] 田维敏[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737

出　　处：《热带作物学报》2016年第7期1312-1317,共6页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院橡胶研究所基本科研业务费(No.1630022015010);国家天然橡胶产业技术体系(No.CARS-34-GW1)

摘　　要：MYC是b HLH转录因子家族的亚家族成员,在植物茉莉酸信号转导过程中发挥着重要的调节作用。Hbl MYC3是从巴西橡胶树的乳管细胞中分离鉴定到的MYC类转录因子,其基因表达受割胶和茉莉酸上调。采用酵母双杂交方法初步筛选Hbl MYC3蛋白的互作蛋白,旨在进一步了解Hbl MYC3的功能。结果表明:Hbl MYC1、Hbl MYC2、DNAJ蛋白、谷氧还蛋白2、含A20和AN1锌指结构域的胁迫相关蛋白5、28 ku热和酸稳定的磷蛋白以及25 ku泛素连接酶E2等7种蛋白不同程度地与Hbl MYC3蛋白互作。基于这些互作蛋白的功能,推测Hbl MYC1或Hbl MYC2通过与Hbl MYC3形成二聚体对小橡胶粒子膜蛋白基因表达进行调控,其他蛋白参与胁迫条件下维持二聚体的稳定性和胁迫反应后降解该二聚体。MYC transcription factors are the members of b HLH-type transcription factor superfamily and play an important role in jasmonate signaling transduction. Available data show that Hbl MYC3 gene is a typical MYC transcriptional factor gene and its transcripts are both enriched in laticifer cells and induced by tapping and exogenous JA. For further revealing its biological functions in laticifer cells, the yeast two-hybridization method was applied to screen candidate interacting partners of the Hbl MYC3. The results showed that Hbl MYC1,Hbl MYC2, DNAJ, glutaredoxin2, zinc finger A20 and AN1 domain-containing stress-associated protein 5, 28 ku heat- and acid-stable phosphoprotein and a 25 ku ubiquitin-conjugating enzyme E2 were detected to interact with Hbl MYC3 to varying degrees. Based on the reported functions of these candidate parterners, Hbl MYC1 or Hbl MYC2 may participate in the regulation of Hbl MYC3 on the SRPP gene expression in a dimer form, while the others contribute to the stability of the dimer during functioning and thereafter the digestion of the dimer through ubiquitin-mediated proteolysis.

关 键 词：巴西橡胶树 Hbl MYC3基因 酵母双杂交 蛋白相互作用 

分 类 号：S794.1[农业科学—林木遗传育种]