外周血单核细胞CD36的表达与类风湿关节炎的相关性研究  

Association between peripheral monocyte CD36 expression and rheumatoid arthritis

作　　者：彭友帆 向阳[1] 韦叶生[1] 钟世茂 

机构地区：[1]右江民族医学院附属医院医学检验科,百色533000

出　　处：《中华风湿病学杂志》2016年第7期441-445,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（81260234,81560552）

摘　　要：目的探讨单核细胞CD36的表达与RA的关系。方法选取2014-2015年在本院就诊的初诊RA患者108例和健康对照108名，采用DAS28-ESR和DAS28-CRP评分系统评价RA患者的疾病活动性，流式细胞术检测单核细胞CD36荧光强度。组间计量资料比较采用两独立样本f检验或Mann-WhitneyU检验。计数资料的组间比较采用x^2检验。变量间的相关分析采用SpeanTlan秩相关，Logistic回归分析与RA相关的风险因素，受试者工作特征（ROC）曲线评价相关变量对RA的评估价值。结果在RA患者和健康对照组中，2组LDL—C[（2．8±0．8）mmollL，（2．6±0．6）mm01／L，t=-2．046，P=-0．042]、ESR[25．5（18．00，40．00）mm／1h，10．00（4．60，10．00）mm／1h，Z=-12．786，P〈0．01]、CRP[15．9（6．71，25．43）mg／L，2．28（1．63，4．60）mg／L，Z=-7．980，P〈0．01]以及单核细胞CD36的表达[（0．44±0．22）×10^9／L，（0．81±0．26）×10^9／L，t=11．159，P〈0．01]差异有统计学意义。在中等活动组和高活动组的RA患者中，单核细胞CD36的表达与其疾病活动性负相关（r=-0．146，P=0．036；r=0．259，P=0．007）。Logistic回归分析结果显示，单核细胞CD36平均荧光强度与RA相关[OR=0．803，P=0．002，95％CI（0．791，0．820）]。单核细胞CD36荧光强度对RA患者评估价值的ROC曲线下面积为0．855195％CI（0．807，0．904），P〈0．01]，敏感度为80．6％，特异度为73．1％。结论单核细胞CD36的平均荧光强度与RA存在密切联系，在活动期的RA患者中与疾病活动相关。Objective To investigate the association between peripheral monocyte CD36 expression and rheum-atoid arthritis （RA）. Methods A total of 108 patients with RA and 108 healthy individuals were included between 2014 and 2015. Disease Activity Score （DAS）28-erythrocyte sedimentation rate （ESR） and DAS28-C reactive protein （CRP） were used to estimate disease activity in patients with RA, and monocyte CD36 fluorescence intensity was determined by using flow cytometry. Student＇s t test or Mann-Whitney U test was used to compare the differences between the two groups. The categorical data was compared using X^2 test. Correlations between variables were analyzed with Spearman correlation, Logistic regression analysis was used to identify risk factors associated with RA, we used receiver operating characteristic （ROC） curve to estimate the performance of relevant variables for RA. Results Low density lipoprotein cholesterin （LDL-C） [（2.8±0.8） mmol/L, （2.6±0.6） mmol/L, t=-2.046, P=0.042], ESR[25.5（18.00, 40.00） mm/1 h, 10.00（4.60, 10.00） mm/1 h, Z=-12.786, P〈0.01], CRP [15.9 （6.71, 25.43） mg/L, 2.28 （1.63, 4.60） mg/L, Z=-7.980, P〈0.01] and monoeyte CD36 expression [（0.44±0.22）×10^9/L, （0.81±0.26）×10^9/L, t=11.159, P〈0.01] was statistically different between the two groups. Of note, monocyte CD36 fluorescence intensity was negatively correlated with disease activity in patients with moderate and severe disease （r=-0.146, P=0.036; r=-0.259, P=0.007）. In stepwise Logistic regression analysis, monocyte CD36 fluorescence intensity was independently associated with RA [OR=0.803, P=0.002, 95%CI （0.791, 0.820）]. ROC curve analysis showed a significant performance with area under curve of 0.855 [95%CI （0.807, 0.904）; P〈0.01], the sensitivity and specificity were 80.6% and 73.1%, respectively, the cutoff values was 0.570. Conclusion Monocyte CD36 fluorescence intensity was independently associated with RA in stepwise Logistic regression analysis. A cor

关 键 词：关节炎 类风湿 单核细胞CD36 疾病活动性 

分 类 号：R593.22[医药卫生—内科学]