SD大鼠胰腺组织在聚焦超声消融损伤后的血管生成  

Angiogenesis after focused ultrasound ablation on pancreas of SD rats

作　　者：敖澜 刘清[1] 胡娜[1] 赵紫豪 胡海霞[2] 方廖琼[1,2] 

机构地区：[1]西南大学生物技术学院,重庆400716 [2]重庆医科大学生物医学工程学院省部共建超声医学工程国家重点实验室超声医学工程重庆市市级重点实验室,重庆400016

出　　处：《中国介入影像与治疗学》2016年第8期495-499,共5页Chinese Journal of Interventional Imaging and Therapy

摘　　要：目的 通过检测经聚焦超声（FUS）消融的SD大鼠胰腺组织凝固性坏死区中Tie-2受体mRNA的表达和组织学结构变化,探讨FUS消融对凝固性坏死组织的血管生成的影响。方法 随机将4~6周龄的SPF级SD大鼠60只分为FUS组（50只）,对照组（10只）。FUS局部消融SD大鼠胰腺组织后,分别取消融后1周,2周,1个月,3个月,6个月和对照组的胰腺组织。用RT-PCR的方法,以GAPDH基因为内参,进行半定量分析检测Tie-2mRNA的表达;同时HE染色、光学显微镜下观察凝固性坏死区组织学的变化;免疫组织化学方法检测胰腺组织中Tie-2的表达。结果 RT-PCR检测到Tie-2mRNA在对照组和FUS消融组织中均有表达。与对照组相比,FUS消融后1、2周Tie-2mRNA的表达量降低（P〈0.01）,消融后1个月mRNA表达量增多,于消融后3个月达到最大值（P〈0.01）,消融后6个月表达量逐渐降低,但仍高于对照组（P〈0.01）。组织学表明,FUS消融后在凝固性坏死区有新生血管的逐步形成。免疫组织化学表明,在正常的胰腺组织中有Tie-2的表达,但在FUS消融胰腺组织中Tie-2的表达增多。结论 在FUS消融后6个月内,大鼠胰腺凝固性坏死区域内有新生血管的生成,且比正常血管生成速度快。Objective To evaluate the effect of angiogenesis of coagulation necrosis tissue and the Tie-2 expression after focused ultrasound （FUS） ablation on the pancreas of SD rats by detecting the expression of Tie-2 mRNA and the histologic structure changes. Methods Totally 60 healthy SD rats of 4 to 6 weeks age were randomly divided into FUS group （n= 50） and control group （n= 10）. Ablated pancreatic specimens were obtained at 0 （normal pancreas）, the 1st week, the 2nd week, the 1st month, the 3rd month and the 6th month after FUS focal ablation for RT-PCR, histology and immunohisto- chemical analysis. The Tie-2 mRNA expression was detected by half quantitative analysis using RT-PCR method. Pancre- atic tissue was dyed with HE stain and examined pathologically under an optical microscope for the coagulation necrosis area. Furthermore, the expression of Tie-2 was observed by immunohistochemistry. Results Expression of Tie-2 mRNA was detected in normal pancreatic tissue and the abated tissues by RT-PCR. Compared with the control group, expression of Tie-2 decreased on the 1st and 2nd weeks after FUS ablation （P〈0. 01）, but mRNA expression increased on the 1st month and reached a maximum on the 3rd month （P〈0. 01）, and the expression gradually reduced on the 6th month after FUS ablation, but the level was still higher than the control group. Histology showed that formation of new blood vessels were observable at the ablation area after the treatment. Immunohistochemically, Tie-2 was expressed in the pre-existing vessels of the normal pancreatic tissue, and Tie-2 ex- pression increased in the ablated pancreatic tissue. Conclusion In the period of the 6 month after FUS ablation, there are new blood vessels formation in the coagulation necrosis area of rats＂ pancreas and it is associated with the expression of Tie-2.

关 键 词：血管生成 超声 聚焦 胰腺 大鼠 Sprague-Dawley 

分 类 号：R454[医药卫生—治疗学]