基于^1H NMR给药赭石后大鼠尿液的代谢组学分析  

1 H NMR-Based Metabonomic Study on Urine from Haematitum-Treated Rats

作　　者：魏莹[1,2] 万传玲 薛蓉[1] 李晓晶[1] 章文军[2] 裴奉奎[1] 

机构地区：[1]中国科学院合成橡胶重点实验室,中国科学院长春应用化学研究所,长春130022 [2]河北工业大学化工学院,天津300130

出　　处：《分析化学》2016年第6期857-863,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.20975097,21305134)资助~~

摘　　要：基于1H NMR的代谢组学方法结合多变量数据分析方法（主成分分析和偏最小二乘判别分析）对灌胃给药赭石（2,5和10 g/kg体重剂量）的成年Wistar大鼠尿液进行分析,并对大鼠给药前1天、给药后1~5天尿液1H NMR数据进行单变量比较分析,筛选出赭石的潜在特征代谢物,对赭石引起的代谢变化进行研究,为赭石的科学用药提供依据。结果表明,大鼠体内柠檬酸、牛磺酸、肌酸酐、α-酮戊二酸、琥珀酸、二甲基甘氨酸等代谢物浓度发生明显变化,随给药时间的变化出现恢复趋势,且恢复趋势与给药剂量相关,可作为赭石的潜在特征代谢物。给药2,5和10 g/kg体重剂量赭石降低了大鼠机体三羧酸循环能力,影响了能量、肌酸及二甲基甘氨酸的代谢,且10 g/kg体重剂量赭石对大鼠肝功能造成一定影响。Wistar rats were intragastrically administered with different doses （2, 5 and 10 g / kg body weight） of haematitum. 1H NMR-based metabonomic analysis coupled with multivariate statistical analysis （principal component analysis and partial least squares-discriminant analysis） was used to analyze the metabolic profiles of the urine samples collected from the treated rats. Univariate analysis on the 1H NMR spectra of urine （1 d before administration, 1-5 d post administration） was used to screen out the potential features of haematitum. Significant treatment related changes were observed for the levels of citrate, tuarine, creatinine,α-ketoglutarate, succinate and dimethylglycine, which could be used as potential features of haematitum. A trend of recovery in connection with dose levels was observed overtime. Such biochemical changes indicated that haematitum treatment at the dose of 2, 5 and 10 g / kg body weight affected the Krebs cycle and glucose metabolism, energy metabolism, choline metabolism and dimethylglycine metabolism in rats. These changes may attribute to the disturbances of hepatic function in 10 g / kg body weight group.

关 键 词：核磁共振 代谢组学 主成分分析 偏最小二乘判别分析 赭石 

分 类 号：R285.5[医药卫生—中药学]