三种方法检查判断血尿来源的比较研究  

A comparative study of three methods of examination to determine the source of hematuria

作　　者：杨麦贵[1] 杨铮[2] 杨阳[3] 岳波[4] 郑善銮[1] 饶国洲[5] 何娟[1] 樊爱琳[1] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院全军临床检验医学研究所,陕西西安710032 [2]第四军医大学学员旅研究生大队,陕西西安710032 [3]第四军医大学西京医院全军整形外科研究所及252医院,陕西西安710032 [4]第四军医大学西京医院耳鼻咽喉头颈外科,陕西西安710032 [5]西安交通大学口腔医院口腔医学研究中心,陕西西安710004

出　　处：《中国实验诊断学》2016年第6期963-965,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨三种检测方法判断血尿来源的比较研究。方法采用显微镜检查红细胞形态变异率、免疫荧光染色和红细胞电泳,分别对318例经临床及病理确诊的血尿来源患者,进行检测比较。结果确诊的208例肾源性血尿红细胞形态学检查总符合率为69.2%(208/144);Ig免疫荧光符合率为98.1%(208/204);红细胞电泳时间符合率为97.6%(208/203),免疫荧光和电泳法符合率明显高于红细胞形态学有显著性差异(P<0.01),免疫荧光与电泳法无明显差异(P>0.05)。三种方法对110例非肾源性血尿检测阴性符合率分别为97.3%(110/107);98.2%(110/108);97.3%(110/107),红细胞形态学法明显高于免疫荧光和电泳法具有显著性差异(P<0.01)。结论肾源性血尿红细胞被免疫球蛋白(Ig)包裹,表面电荷降低,使Ig免疫荧光和红细胞电泳法对诊断肾源性血尿较特异准确,红细胞形态学虽较差,但易开展适于各级医疗单位,对非肾源性血尿诊断率优于免疫荧光和电泳法。Objective Discussion comparative study to determine the source of the three detection methods hematu- ria. Methods Microscopic examination of the use of red blood cell morphology mutation rate,immunofluorescence staining and erythrocyte electrophoresis, respectively, 318 cases diagnosed by clinical and pathological origin of hematuria patients were detected compare. Results Confirmed 208 cases of renal hematuria RBC morphology total coincidence rate 69.2% （208/144） ;Ig immunofluorescence coincidence was 98.1% （208/204） ;erythrocyte electrophoresis time the rate was 97.6 % （208/203） ,immunofluorescence and electrophoresis was significantly higher red blood cell morphology consistent with a significant difference（P〈0.01）, no significant difference immunofluorescence and electrophoresis （P〉0.05）. Three methods of 110 cases of non-compliance with renal hematuria negative detection rates were 97.3% （110/107） ;98.2% （110/108）;97.3% （110/107）, significantly higher red blood cell morphology law immunofluores-eence and electrophoresis was a significant difference（P〈0.01）. Conclusion RBC renal hematuria is an immunoglobulin （Ig） parcels,the surface charge reduced,so Ig immunofluorescence and erythrocyte electrophoresis method for the diagnosis of renal hematuria more specific and accurate, though less red blood cell morphology, but easy to carry suitable for all levels medical units,for non-renal hematuria diagnostic yield than immunofluorescence and electrophoresis.

关 键 词：血尿-来源 红细胞 形态学 免疫荧光 电泳-时间 

分 类 号：R446.12[医药卫生—诊断学]