牙周局部炎症影响小鼠肠道机械及免疫屏障功能  

Periodontal inflammation affects the mechanical and immune barrier functions of mice gut

作　　者：谢美莲[1] 余挺[2] 卓颖[1] 黄馨[1] 谢宝仪[1] 轩东英[1,3] 章锦才[1,3] 

机构地区：[1]南方医科大学附属口腔医院牙周科,广州510280 [2]广州医科大学附属口腔医院牙周科,广州510150 [3]中国科学院大学存济医学院附属杭州口腔医院牙周科,杭州310006

出　　处：《华西口腔医学杂志》2016年第4期414-418,共5页West China Journal of Stomatology

基　　金：国家自然科学基金(81271160;81371151;81470750)~~

摘　　要：目的探索牙周局部炎症对小鼠肠道屏障(生态屏障、机械屏障、免疫屏障)功能的影响。方法将20只雄性C57BL/6J小鼠随机分为牙周炎组(P组)和对照组(C组),每组10只。P组使用浸泡牙龈卟啉单胞菌的丝线行牙周结扎10 d,C组假性结扎。分离上颌骨测量牙槽骨丧失量,取肠道内容物行16s r RNA焦磷酸测序分析菌群结构,免疫组织化学检测回肠组织occludin、claudin2、NOD2蛋白的表达。结果 P组的牙槽骨丧失量高于C组(P<0.001)。P组与C组的主要菌门及卟啉单胞菌种比例无统计学差异(P>0.05),P组occludin、claudin2、NOD2的表达高于C组(P=0.039,P=0.011,P=0.039)。结论牙周局部炎症在一定程度上影响了小鼠肠道的机械及免疫屏障功能。Objective To explore the effects of periodontal inflammation on the functions of gut barrier （ecological barrier, mechanical barrier, and immune barrier） in mice.Methods Twenty male C57BL/6J mice were randomly divided into perio-dontitis （P） or control （C） groups. The P group was subjected under a 10-day ligation withPorphyromonas gingivalis to induce periodontitis, whereas the C group was ligated with sham. Maxillae were obtained to assess alveolar bone loss. The phyloge-netic structure and diversity of microbial communities in the gut were analyzed by 16s rRNA pyrosequencing. Immunohisto-chemical analysis was performed to determine the expressions of occludin, claudin2, and NOD2 in the ileum.Results Com-pared with the C group, the P group displayed significant alveolar bone loss （P〈0.001）. In addition, no significant influence on the main phyla and genus Parabacteroides of the two groups was observed （P〉0.05）. However, the ileum of the P group showed significantly upregulated occludin, claudin2, and NOD2 （P=0.039,P=0.011, andP=0.039, respectively）.Conclusion Periodontal inflammation influences to some extent the mechanical and immune barrier functions of the mice gut.

关 键 词：牙周炎 炎症性肠病 肠道菌群 机械屏障 

分 类 号：R781.4[医药卫生—口腔医学]