鱼类肠道铁载体高产菌株CB-EH-2的筛选与鉴定  

Screening and Identification of High Sidero-Carrier High-Producing Bacteria CB-EH-2 from Intestinal Tracts of Marine Fish

作　　者：李红芳[1] 翟秋倩 门晋名[1,2] 韩晓红[1] 刘丽[1] 李俊峰[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042 [2]迪沙药业集团,山东威海264200

出　　处：《微生物学杂志》2016年第3期32-38,共7页Journal of Microbiology

基　　金：山东省高校科技计划项目(J14LE59);青岛市应用基础研究计划项目(12-1-4-3-(3)-jch);山东省科技发展计划项目(2015GSF121030)

摘　　要：利用2216E限铁培养基从青岛近海海鱼肠道内筛选到1株铁载体高产菌株,命名为CB-EH-2。结合形态、生理生化特性、16S rRNA基因序列同源性和系统发育分析将其初步鉴定为Cobetia amphilecti。铬天青(CAS)法检测其铁载体的产生及对Fe3+的亲和能力。XAD-2大孔径树脂提取铁载体,铁载体类型和拮抗活性分析显示,菌株CB-EH-2所产铁载体为异羟肟酸型,可显著抑制鳗弧菌等4种指示菌的生长。不同浓度2,2'-联吡啶及铁离子对CB-EH-2的菌体量和铁载体产量具有较大的影响。本文首次报道C.amphilecti产生异羟肟酸型铁载体,CB-EH-2作为有益菌值得进一步研究。A high sidero-carrier high-producing bacterial strain was screened from the intestinal tracts of marine fishusing iron-limited 2216E medium, and named as CB-EH-2, initially identified as Cobetia amphilecti with morphologi-cal, physiological, and biochemical features, 16S rRNA gene sequence homology and phylogenctics analysis. Thechrome azurol S （CAS） assay was used to detect the sidero-caiTier production and its affinity to Fe3＋. Sidero-carrierproduced by CB-EH-2 were isolated from the cell-free culture supernatant by adsorption to Amberlite XAD-2 resin.Antagonistic activity and type of sidero-carrier from CB-EH-2 were analyzed. The results show that CB-EH-2 producedhydroxamate-type sidero-carrier against four indicator stains including Staphylococcus aureu3, E. coli, Vibrio anguilla-rum and Vibrio splendidus. Different concentrations of 2, 2＇-bipyridine or Fe3＋ have a great influence on the growthand the sidero-carrier production of CB-EH-2. C. amphilecti was the first reported to produce hydroxamate-sidero-car-rier, and CB-EH-2 was worthy to study further as probiotic.

关 键 词：Cobetiaamphilecti 铁载体 筛选 鉴定 

分 类 号：Q939.1[生物学—微生物学]