通络救脑口服液对快速老化模型小鼠RAGE和Aβ的影响  

Influences of Tongluo Jiunao Oral Liquid on RAGE and Aβ in Rapid Aging Mice

作　　者：高爱社[1] 杜欢[2] 范英昌[2] 王榭延 

机构地区：[1]河南中医药大学,河南郑州450008 [2]天津中医药大学,天津300193

出　　处：《中药新药与临床药理》2016年第4期533-536,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金青年基金项目(81303088);河南省科技厅基础与前沿科研项目(142300410171)

摘　　要：目的探讨通络救脑口服液对快速老化模型(SAMP8)小鼠晚期糖基化终末产物受体(RAGE)和淀粉样蛋白β(Aβ)表达的影响。方法 24只SAMP8小鼠随机分为模型组、通络救脑高、低剂量组(9,6 m L·kg^(-1)·d^(-1)),另设抗快速衰老亚系1(SAMR1)小鼠对照组(正常对照组),连续灌胃给药21 d。取海马区脑组织,分别采用酶联免疫吸附法(ELISA)和免疫组织化学法检测小鼠大脑海马内Aβ以及RAGE的表达。结果与模型组比较,通络救脑高、低剂量组海马区脑组织RAGE和Aβ表达降低(P<0.01),RAGE蛋白含量明显降低(P<0.01)。结论通络救脑口服液可通过抑制海马区脑组织RAGE和Aβ的表达,以减轻RAGE和Aβ介导的神经病理损伤作用。Objective To observe the influences of Tongluo Jiunao Oral Liquid（TLJN） on receptor for advanced glycation endproducts（RAGE） and the amyloid-β（Aβ） deposits in a senescence accelerated mouse prone-8 strain（SAMP8） model. Methods We used male senescence accelerated mouse prone-8 strain（SAMP8） mice that were randomly divided into model,TLJN min,TLJN max,and senescence-accelerated resistant mouse（SAMR1）that we used as a control group. The Aβ deposits were measured using immunohistochemistry. The expression of RAGE were measured using ELISA and immunohistochemistry. Results Compared with model group, TLJN-treated groups exhibited a reduction of Aβ deposits,and TLJN effectively attenuated Aβ-induced expression of RAGE. Conclusion TLJN can attenuate neural injury probably by inhibiting RAGE and Aβ expression in the pathogenesis of AD.

关 键 词：通络救脑口服液 淀粉样蛋白β 晚期糖基化终末产物受体 快速老化小鼠 

分 类 号：R285.5[医药卫生—中药学]