小鼠诱导多能干细胞与PHBHHx膜体外共培养的实验研究  

Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body

作　　者：许士俊 穆军升[1] 张健群[1] 伯平[1] 

机构地区：[1]首都医科大学附属北京安贞医院北京市心肺血管疾病研究所心外科,北京100029

出　　处：《中国胸心血管外科临床杂志》2016年第8期822-826,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：2014年度北京市朝阳区科技计划项目(SF1417)~~

摘　　要：目的探讨小鼠诱导多能干细胞(mi PSCs)与聚3-羟基丁酸-3-羟基己酸共聚物(PHBHHx)膜材料的体外相容性。方法复苏mi PSCs,培养传代后,分为两组,一组与PHBHHx膜材料在体外共培养,通过DAPI染色方法观察mi PSCs在膜上的粘附情况,细胞计数试剂盒-8(CCK-8)测定mi PSCs在PHBHHx膜上的增殖,扫描电镜观察细胞与膜材料的复合情况;另一组用传统的培养皿培养mi PSCs,CCK-8测定细胞活力。结果小鼠诱导多能干细胞能够与PHBHHx膜材料良好的粘附和增殖,CCK-8实验测定细胞活力OD值结果显示PHBHHx膜显著大于传统的细胞培养皿培养(0.617±0.019 vs.0.312±0.004,P<0.05)。结论 PHBHHx与mi PSCs体外共培养制成的细胞补片表面上有细胞粘附及增殖,与传统的细胞培养皿培养相比,PHBHHx膜培养在细胞的粘附和增殖中具有很大的优势,PHBHHx可作为干细胞治疗多种疾病的支架材料之一。Objective To study the external biocompatibility bewteen the mouse induced pluripotent stem cells （miPSCs） and poly-3-hydroxybutyrate-co-3-hydroxyhexanoate （PHBHHx）. Methods After we recovered and subcultured miPSCs, we divided them into two groups. There was one group cultured with material of PHBHHx films outside the body. We observed the adhesive pattern of miPSCs on film by fluorescence of 4,6-diamidino-2-phenylindole （DAPI） staining. The cell vitality was detected by cell counting kit-8 （CCK-8）. The morphology of miPSCs attached on the film was visualized under scanning electron microscope （SEM）. We used the traditional petri dish to culture miPSCs and detect the cell activity by CCK-8. Results MiPSCs can adhere and proliferate on PHBHHx films. The result of cell vitality which detected by CCK-8 showed that there was a statistical difference in OD value between culturing on PHBHHx films and traditional cultivation （0.617±0.019 vs. 0.312±0.004, P〈0.05）. Conclusion There are adhesion and proliferation on the surface of cells patch made by miPSCs co-culturing with PHBHHx film. Compared with traditional culturing in the cell culture dish, culturing in PHBHHx films have great advantages in the process of adhesion and proliferation. PHBHHx can be used as one of the scaffold for stem cells treating various disease.

关 键 词：小鼠诱导多能干细胞 细胞补片 聚3-羟基丁酸/3-羟基己酸共聚物 干细胞治疗 

分 类 号：R318.08[医药卫生—生物医学工程]