Multiple Resistance to Acetyl Coenzyme A Carboxylase and Acetolactate Synthase Inhibiting Herbicides in Tunisian Ryegrass Populations （Lolium rigidum）  

作　　者：Haifa Hajri Yosra Menchari Abdelwahed Ghrobel 

机构地区：[1]Center of Biotechnology of Borj Cedria, University of Tunis El Manar, BP 901 Hammam-Lif 2050, Tunisia [2]Higher Institute of Biotechnology of Beja, University of Jendouba, Avenue Habib Bourguiba, BP 382, Beja 9000, Tunisia

出　　处：《Journal of Agricultural Science and Technology(A)》2015年第9期738-744,共7页农业科学与技术（A）

摘　　要：The good understanding of the mechanisms of resistance to herbicides in weeds is a necessity to implement sustainable weed management strategies. Here, a study was conducted to characterize the molecular bases of resistance to acetyl coenzyme A carboxylase （ACCase） and acetolactate synthase （ALS） inhibiting herbicides in Lolium rigidum populations from Tunisia. Nine Lolium rigidum （ryegrass） populations collected in wheat fields from Northern Tunisia were investigated for their resistance to two ACCase-inhibiting herbicides and an ALS-inhibiting herbicide. All populations were tested in the greenhouse in pots using the commercial dose to determine resistance status. Survival plants were also tested for the presence of two ACCase （L 1781 and N2041） and two ALS （P197 and W574） mutant resistant alleles using molecular markers. Resistance to ACCase-inhibiting herbicides was found in all tested populations. Comparison of the results from herbicide sensitivity bioassays with genotyping indicated that more than 80% of the plants resistant to ACC-inhibiting herbicides would be resistant via increased herbicide metabolism. However, ALS-inhibiting herbicides are still more or less controlling ACCase resistant populations, so indicating that the selection process of resistance is ongoing. Target-site resistance appears to be the major mechanism for these early cases of ALS inhibitor resistance. This study reported the first case of resistance to ALS-inhibiting herbicides in ryegrass in Tunisia, and investigated the molecular bases of this resistance. It establishes the clear importance of non target-site resistance to ACCase- and/or ALS-inhibiting herbicides.

关 键 词：ACCASE ALS cross resistance LOLIUM mutant resistant alleles metabolism. 

分 类 号：S482.4[农业科学—农药学] S544.9[农业科学—植物保护]