小型动物连续性动脉-静脉血液滤过血液净化平台的构建  

Establishment of blood purification system for rats

作　　者：俞雯艳[1] 马帅[1] 徐庆青 邓博[1] 方均燕[1] 刘婷彦 丁峰[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院肾脏科危重肾脏病研究室,200011

出　　处：《中华肾脏病杂志》2016年第7期507-512,共6页Chinese Journal of Nephrology

基　　金：国家自然科学基金（81070609、81270850、81470990）;上海市科委重点项目（11441901401）;国家科技支撑项目（2011BA110808）;上海市浦东新区卫计委联合攻关项目（PW2015D-4）;上海交通大学医工交叉重点项目（YG2014ZD06）

摘　　要：目的：构建小型动物血液净化系统，为血液净化技术治疗机制的基础研究提供安全、可靠的实验平台。方法选用雄性SD大鼠，使用PE50插管开放右侧颈动脉和对侧颈静脉作为体外循环的血管通路，建立连续性动脉？静脉血液滤过血液净化系统。血流速度、置换液速度和超滤速度均由小型蠕动泵控制在一定范围内。在维持麻醉和肝素抗凝的基础上进行4h血液净化治疗。检测血液净化治疗过程中动脉血气、电解质及血糖的变化情况；监测血压、心率等生命体征；观察血液净化治疗过程中是否发生大量出血、血栓形成及气体栓塞等不良反应。结果动脉血气、电解质及血糖未发生明显变化（P＞0.05）；血液净化治疗前后生命体征未发生明显波动（P＞0.05）；血液净化治疗过程中不良反应发生率低。结论动脉？静脉血液滤过对健康大鼠未造成明显不良反应，小型动物血液净化平台安全、可行。Objective To establish blood purification system for rats and provide a safe and reliable experimental platform for further research of blood purification. Methods The right carotid artery and the contralateral jugular vein of adult male Sprague？Dawley rats were cannulated to creat vascular access for blood purification, by which continuous arteriovenous hemofiltration blood purification system was established. Blood flow, substitution fluid flow and ultrafiltration rate were regulated by rotary mini？pumps. Blood purification therapy continued for 4 hours on the basis of maintained anesthesia and effective anticoagulation. The safety of continuous arteriovenous hemofiltration blood purification systems was evaluated by comparing the arterial blood gas, electrolyte indexes and blood glucose during the blood purification therapy. Closely monitoring the vital signs of rats, such as blood pressure and heart rate, and observing whether there were any side effects, such as massive haemorrhage, thrombogenesis and gas embolism in the therapeutic process. Results There were no obvious changes of arterial blood gas, electrolyte indexes and blood glucose during the blood purification therapy （P〉0.05）. The vital signs did not fluctuate acutely before and after the blood purification therapy （P〉0.05）. The incidence rate of side effects was very low. Conclusions 〈br〉 Continuous arteriovenous hemofiltration blood purification system had no obvious adverse effects on healthy rats. Our blood purification system for rats appears to be safe and reliable.

关 键 词：模型 动物 大鼠 SPRAGUE-DAWLEY 血液滤过 血液净化 

分 类 号：R459.5[医药卫生—治疗学]