骨髓间充质干细胞对脂多糖诱导的足细胞损伤的影响  

Effects of bone marrow-derived mesenchymal stem cells on glomerular podocyte injured by lipopolysaccharide

作　　者：陈怡[1] 柳林芬 万建新[1] 崔炯[1] 尤丹瑜[1] 邹臻寰[1] 

机构地区：[1]福建医科大学附属第一医院肾内科,福州350005

出　　处：《中华肾脏病杂志》2016年第7期528-533,共6页Chinese Journal of Nephrology

基　　金：福建省自然科学基金（2014J01314）

摘　　要：目的：观察骨髓间充质干细胞（BMSC）对脂多糖（LPS）诱导的足细胞损伤的影响。方法将足细胞分为正常条件培养足细胞（Control）组、足细胞与BMSC共培养（BMSC）组、LPS诱导（LPS）组、LPS诱导＋BMSC共培养（LPS＋BMSC）组。不同实验条件下干预24 h后，观察足细胞形态改变，用RT？PCR法和Western印迹方法分别检测足细胞相关蛋白nephrin、CD2AP、synaptopodin和TRPC6 mRNA及蛋白的表达。结果与Control组比较，LPS组足细胞nephrin、synaptopodin、CD2AP mRNA和蛋白的表达水平明显降低（均P＜0.05），TRPC6 mRNA和蛋白的表达水平明显增加（P＜0.05）；与LPS组比较，LPS＋BMSC组足细胞nephrin、CD2AP、synaptopodin mRNA和蛋白的表达水平明显升高（均P＜0.05），TRPC6 mRNA和蛋白的表达水平明显下降（P＜0.05）。结论 BMSC可以减轻LPS诱导的足细胞的损伤。Objective To observe the effects of bone marrow？derived mesenchymal stem cells （BMSC） on glomerular podocyte injured by lipopolysaccharide （LPS） and the expression of related protein. Methods Podocytes are divided into control group, BMSC group, LPS group and LPS plus BMSC group. After 24 hours of intervention, observing each experimental group podocyte form under inverted phase contrast microscope;detecting the expressions of mRNA and protein of nephrin, CD2AP, synaptopodin, and TRPC6 by RT？PCR and Western？blot. Results Compared with control group, expressions of nephrin, CD2AP, and synaptopodin in LPS group decreased （P〈0.05） while that of TRPC6 increased （P〈0.05）; compared with LPS group, expressions of nephrin, CD2AP, and synaptopodin in LPS＋MSC group increased （P〈0.05） while that of TRPC6 decreased （P〈0.05）. Conclusion BMSC may relieve LPS？induced podocyte injury.

关 键 词：足细胞 间质干细胞 脂多糖类 共培养 

分 类 号：R692[医药卫生—泌尿科学]