水飞蓟宾对金黄色葡萄球菌和大肠杆菌的体外抑菌作用的研究  

In Vitro Bateriostasis of Silibinin against Escherichia Coli and Staphlococcus Aureus

作　　者：杜文静[1] 谢亚斌[1] 葛雅琨[1] 张元新[1] 

机构地区：[1]吉林化工学院生物与食品工程学院

出　　处：《吉林化工学院学报》2016年第7期66-69,共4页Journal of Jilin Institute of Chemical Technology

摘　　要：水飞蓟宾是来源于菊科植物水飞蓟中的一种黄铜木质素类化合物,具有明显的抗氧化、抗炎、抗肿瘤等特性.通过SDA-PAGE和碱性磷酸酶实验分析水飞蓟宾作用前后的菌体蛋白质及碱性磷酸酶的变化,研究其对金黄色葡萄球菌和大肠杆菌的抑菌效果的差异.研究结果表明,水飞蓟宾是通过破坏细胞壁结构、抑制菌体对蛋白质的利用,导致金黄色葡萄球菌正常生理功能的丧失或减退,达到抑菌的目的;而对大肠杆菌的作用不敏感.Silibinin, flavonolignans extracted from Silybum marianum, has obvious antioxidant, anti-inflammatory,anti-tumor properties.Though SDS-PAGE and alkaline phosphatase assay,we analyze of protein and activity of alkaline phosphatase and investigate the difference of the bacteriostatic Silibinin treatment of Staphylococcus aureus and Escherichia coli.The results show that Silibinin growth and protein utilization of the Staphlococcus Aureus through the disruption of cell wall and the change effect after inhibit the the loss of physiological function of bacterium.Escherichia coli is not sensitive to the silibinin.

关 键 词：水飞蓟宾 抑菌作用 金黄色葡萄球菌 大肠杆菌 

分 类 号：Q501[生物学—生物化学]