柴油降解菌的筛选鉴定和降解特性研究  

Screening of Diesel Oil Degradable Microorganisms and Characterization of the Diesel Oil Degradation

作　　者：赵海乐[1] 温钢[2] 

机构地区：[1]吉林化工学院 [2]吉林化工学院生物与食品工程学院,吉林吉林132022

出　　处：《吉林化工学院学报》2016年第7期70-77,共8页Journal of Jilin Institute of Chemical Technology

基　　金：国家自然科学基金(No.41302185)

摘　　要：试验介绍了一种高效降解柴油菌株,经生理生化试验与16S rDNA序列分析等对该菌株进行鉴定.采用单因素试验,确定最优生长条件,即以1 g/L NH_4Cl为氮源、5 g/L NaCl为盐度、温度30℃、pH6.0、接种量20%.在此最佳条件下培养时间72h后测得该菌株的柴油降解率为29.29%.In this paper, a new strain that have a efficient diesel degradation was introduced. The strain was identified through the analyses of physiological, and biochemical characteristics and 16S rDNA sequence.The optimum growth conditions are 1 g/L NH4Cl as nitrogen source,5 g/L NaCl as salinity,temperature of 30℃, pH of 6.0, inoculation amount of 20% and growth time of 72 h by the single-factor test. Under the optimal growth conditions ,the diesel degradation by the strain could reach 29.29%.

关 键 词：0^#柴油 生理生化试验 最佳生长条件 降解率 

分 类 号：X172[环境科学与工程—环境科学]