比较含红芪和含黄芪的补中益气汤含药血清对SAMP8鼠脾淋巴细胞抗免疫老化的作用  

Effects of serums containing Buzhong Yiqi decoction with Astragalus Radix or Hedysari Radix on anti-immunosenescence in spleen lymphocytes of SAMP8 mice

作　　者：张高林 程卫东[2,3] 张文君[4] 李纬[2] 李钦 

机构地区：[1]甘肃省中医学校,甘肃兰州730050 [2]南方医科大学中医药学院,广东广州510515 [3]兰州大学基础医学院,甘肃兰州730000 [4]甘肃省中医院,甘肃兰州730050

出　　处：《中国中药杂志》2016年第15期2888-2894,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373806);甘肃省中医药管理局课题(GZK-2013-18)

摘　　要：比较研究含红芪和黄芪补中益气汤对快速老化小鼠8(SAMP8)脾淋巴细胞免疫功能的影响。通过MTT法检测Con A诱导SAMP8鼠脾脏淋巴细胞增殖能力的影响。流式细胞术检测含药血清对T淋巴细胞亚群分化百分比的影响。ELISA法检测培养上清液中IL-2和IFN-γ的表达。qRT-PCR法检测含药血清对T淋巴细胞CD28 mRNA表达的影响,蛋白印迹法检测SAMP8鼠脾脏CD28蛋白的表达含量。含红芪和含黄芪的补中益气汤含药血清均能提高SAMP8鼠T淋巴细胞的增殖能力;对SAMP8小鼠T淋巴细胞亚群的分化作用不明显,没有统计学差异;均可促进培养上清液中IL-2和INF-γ的分泌,且分泌IL-2水平,补红组优于补黄组(P<0.05);均能增加T淋巴细胞中CD28 mRNA的表达量,且红芪组优于黄芪组(P<0.05);均能提高SAMP8鼠脾脏CD28蛋白的表达,且红芪组与黄芪组无明显差异。含红芪和黄芪的补中益气汤含药血清可以通过提高脾淋巴细胞的增殖和增加CD28分子百分比的表达发挥抗免疫老化的作用,在抗免疫衰老方面红芪补中益气汤和黄芪补中益气汤作用相似。This paper was aimed to compare the effect of Buzhong Yiqi decoction containing Hedysari Radix or Astragali Radix on anti-immunoseneseenee effects in spleen lymphoeytes of senescence accelerated mouse 8 （SAMPS）. The effect of the serums on the proliferation of spleen T lymphocytes in SAMP8 mice induced by ConA was tested by MTT. The effect of the serums on the T lympho- cytes subsets of SAMP8 mice was measured by flow cytometry. ELISA was used to detect the level of IL-2 and IFN-γ in the culture su- pernatants of spleen lymphocytes. The effect of the serums on the expression of CD28 mRNA in spleen T lymphocytes was detected by fluorescent quantitative PCR. Western blot was used to detect the expression of CD28 protein in spleen T lymphocytes of SAMP8 mice. Both the serums of Buzhong Yiqi decoctions containing Hedysari Radix or Astragali Radix improved the proliferation of T lymphoeytes in SAMP8 mice. Both the serums had no obvious effect on the differentiation of spleen T lymphocytes＇subsets in SAMP8 mice. Both the serums increased the content of IL-2 and INF-γ in the culture superuatants of spleen lymphoeytes. And for the content of IL-2, the set-um of Buzhong Yiqi decoction with Hedysari Radix was better（P 〈 0. 05）. Both the serums improved the expression of CD28 mRNA in spleen T lymphocytes of SAMP8 mice. And the effect of Hedysari Radix group was better than that of Astragalus Radix group （ P 〈 0.05 ）. Both the serums improved the expression of CD28 protein in spleen T lymphocytes of SAMP8 mice. The role of the serums con- taining Buzhong Yiqi decoction with Astragalus Radix and the decoction with Hedysari Radix in anti-immunosenescence was through the effect of the CD28. And the effect of Hedysari Radix group was better than that of Astragalus Radix group on improved the expression of CD28 mRNA in T lymphocytes of SAMP8 mice. Astragalus Radix and Hedysari Radix could swap in the aspect of anti-immunosenescence.

关 键 词：红芪 黄芪 补中益气汤 SAMP8小鼠 免疫老化 

分 类 号：R285.5[医药卫生—中药学]