雾化吸入痰热清、喜炎平对大鼠肺部的刺激性研究  

Study on the rat pulmonary irritation of aerosol inhaled Tanreqing and Reduning

作　　者：谭宁[1] 李鹏跃[1] 董博宇[1] 赵梦迪[1] 杨柳[1] 陆洋[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《药学实践杂志》2016年第4期348-350,356,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金项目(81473363);北京市科技新星计划(xx2015A048)

摘　　要：目的研究中药复方痰热清和单成分喜炎平注射液的雾化吸入对大鼠肺部的刺激性。方法每种药物分为高、低浓度2个实验组,对照组灌以生理盐水。雾化吸入药物给药7d后处死大鼠。采用大鼠支气管肺泡灌洗术,测定灌洗液中总蛋白含量和LDH酶活性,以判断两种药物对大鼠肺部的损伤程度。结果痰热清低、高浓度组肺泡灌洗液中蛋白质浓度分别为:(193.78±27.74)、(235.33±50.41)μg/ml;喜炎平两组蛋白质浓度为:(174.02±17.82)、(227.27±66.03)μg/ml;LDH酶活力单位依次为:1 065.21±181.76、1 467.33±101.87;307.97±47.56、1 377.29±566.48。经t检验,与对照组相比,痰热清和喜炎平(低浓度和高浓度)对肺泡灌洗液中总蛋白含量无显著影响,但均能增加LDH酶的活力(P<0.05),且与浓度呈正相关;低浓度痰热清组的LDH酶活力显著高于低浓度喜炎平组(P<0.05)。结论雾化吸入痰热清、喜炎平对大鼠呼吸系统有一定的刺激性,故不宜采用雾化吸入的给药方式。Objective To study the rat pulmonary irritant of aerosol inhaled Tanreqing and Reduning injection.Methods Rats were devided into two groups for each medicine（low concentration group and high concentration group）,nebulized drug administration for seven days,with the control group irrigated with saline,and were sacrificed.Through bronchoalveolar lavage,excurrent bronchoalveolar lavage fluid（BALF）was used for total protein determination and LDH vitality test to evaluate pulmonary toxicity of two medicines.Results The protein concentrations of two groups in low and high concentrations of Tanreqing and Reduning respectively are（193.78±27.74）,（235.33±50.41）μg/ml;（174.02±17.82）,（227.27±66.03）μg/ml;LDH vitalities respectively are 1065.21±181.76,1467.33±101.87;307.97±47.56,1377.29±566.48.By t-test,compared with normal saline,there was no significant effect among these five groups on protein concentration,but these two medicine were able to improve LDH activity（P〈0.05）which was more obvious in high concentration group.When two medicines were in low concentration,LDH activity was higher in Tanreqing group with statistical significance（P〈0.05）.Conclusion Aerosol inhaled Tanreqing and Reduning injection in rats have some pulmonary irritation and potential safety hazard in this delivery way.

关 键 词：肺部给药 痰热清 喜炎平 支气管肺泡灌洗术 刺激性 

分 类 号：R944.7[医药卫生—药剂学]