ELISA与RFFIT两种方法检测人狂犬病抗体的对比  

Comparison of Two Methods of ELISA and RFFIT for Detection of Human Rabies Antibody

作　　者：熊春[1] 

机构地区：[1]长沙市疾病预防控制中心质量管理科,湖南长沙410004

出　　处：《中国卫生标准管理》2016年第13期159-160,共2页China Health Standard Management

摘　　要：目的对比酶联免疫吸附试验(ELISA)与快速免疫荧光灶抑制试验(RFFIT)检测人狂犬病抗体的效果。方法选取狂犬抗体疫苗接种者免疫后血清100份,分别随机选用ELISA与RFFIT检测。结果免疫0 d、3 d、7 d、14 d和45 d时ELISA法检测阳性率分别为9.00%、12.00%、25.00%、48.00%、86.00%;RFFIT法检测阳性率分别为0.00%、0.00%、23.00%、100.00%、100.00%。结论 ELISA与RFFIT两种方法检测人狂犬病抗体均可适用于抗狂犬病毒抗体检测,但ELISA法免疫早期假阳性率较高,后期假阴性率较高。Objective To compare enzyme linked immunosorbent assay （ELISA） and rapid fluorescent focus inhibition test （RFFIT） on the detection of human rabies antibodies.Methods100 cases of serum of rabies antibody vaccine recipients after vaccination were selected and randomly detected with RFFIT or ELISA. Results 0 d,3 d,7 d,14 d and 45 d after vaccination,the positive detection rate by ELISA were respectively 9.00%,12.00%,25.00%, 48.00% and 86.00%. The positive rate by RFFIT were 0.00%,0.00%,23.00%,100.00% and 100.00%.ConclusionBoth ELISA and RFFIT can be applied to the detection of rabies virus antibody,but ELISA has higher false positive rate in early period of immunization and higher false negative rate in later period of immunization.

关 键 词：人 狂犬病 抗体 

分 类 号：R512.62[医药卫生—内科学]