谷氨酸棒状杆菌新型诱导启动子的研究  

Study of the New Inducible Promoter in Corynebacterium Glutamicum

作　　者：范荣[1] 刘金雷[1] 韩武洋 许湄雪 陆浩[1] 仪宏[1] 李天明[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《海南师范大学学报（自然科学版）》2016年第2期154-159,共6页Journal of Hainan Normal University（Natural Science）

基　　金：国家科技支撑计划(2015BAD15B05)

摘　　要：以丰富和完善谷氨酸棒状杆菌的表达元件、筛选适合工业化应用的新型诱导启动子为研究目标,借助绿色荧光蛋白GFP为报告基因,构建启动子筛选工具质粒,在谷氨酸棒杆菌中表达,筛选出三种高效表达的诱导启动子.通过检测绿色荧光蛋白表达强度可知:丙酸启动子诱导表达强度最大,葡萄糖酸启动子次之,蔗糖启动子较弱;丙酸启动子最适诱导浓度为60 ug/m L、葡萄糖酸启动子2 mg/m L、蔗糖启动子2 mg/m L,表达强度比无诱导对照组分别增加4.39、3.86、2.74倍.To enrich the expression elements of Corynebacterium glutamicumand screen out new available inducible promotets in industrial application, the article usesGreen fluorescent protein （GFP）as report system to construct promoter selecting articles and expresses them in Corynebacterium glutamicum. Three highly expressed inducible promoters are selected. By measuring expression levelof Green fluorescent protein, we demonstrate that propionate promoter is the highest of all in induction intensity, gluconate promoter comes the second, and sucrose promoter is the lowest. The propionate concentration of only 60 ug/mL is found to be sufficient for full induction（increasing 4.39 fold）of its native target.Adding 2mg/mL gluconate and sucrose respectively, the expression intensity reaches its maximal induction, increasing 3.86 fold and 2.74 fold than its native target respectively.

关 键 词：谷氨酸棒状杆菌 丙酸诱导启动子 葡萄糖酸诱导启动子 蔗糖诱导启动子 

分 类 号：Q78[生物学—分子生物学]