血红蛋白在多孔ZnO纳米球修饰电极上的直接电化学与电催化  

Direct Electrochemistry of Hemoglobin on Mesoporous ZnO Nanosphere Modified Electrode and Electrocatalysis

作　　者：吴思远[1] 闫丽君[1] 沈青凤 甄超[1] 蒙泽慧 夏智高[1] 雷炳新[1] 孙伟[1] 

机构地区：[1]海南省水环境污染治理与资源化重点实验室海南师范大学化学与化工学院,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2016年第2期184-188,共5页Journal of Hainan Normal University（Natural Science）

基　　金：海南省水环境污染治理与资源化重点实验室开放基金资助课题;海南省自然科学基金(20152016)

摘　　要：以1-丁基吡啶六氟磷酸盐作为粘合剂制备的离子液体修饰碳糊电极为基底电极,将多孔ZnO纳米球和血红蛋白(Hb)用壳聚糖固定在电极表面制备出电化学酶传感器.紫外可见光谱和傅里叶变换红外光谱实验结果表明Hb在复合膜内保持其基本结构.通过修饰电极在PH 6.0的PBS缓冲溶液中的循环伏安,得到了的一对良好的氧化还原峰,可以证明Hb分子结构中的电活性中心与电极之间发生了直接电子转移,对Hb的电化学行为进行研究,求解了相关的电化学参数,该Hb修饰电极对三氯乙酸表现出良好的电催化活性.Ionic liquid 1-butylpyridinium hexafluorophosphate was used as the modifier to prepare a carbon ionic liquid electrode and used as the base electrode. Mesoporous ZnO nanosphere and hemoglobin （Hb） were immobilized on the CILE surface by a chitosan film to get an electrochemical sensor. UV-Vis absorption and FT-IR infrared spectra proved that Hb remained its basic conformation in the composite. Cyclic voltammetric results exhibited a pair of well-defined redox peak in pH 6.0 PBS, which was ascribed to the direct electron transfer of electroactive center of Hb with the working electrode. Electrochemical behaviors of Hb on the modified electrode were studied and the electrochemical parameters were calculated. This Hb modified electrode showed excellent electrocatalytic activity to the reduction of tricholoacetic acid.

关 键 词：血红蛋白 多孔ZnO纳米球 离子液体修饰碳糊电极 直接电化学 电催化 

分 类 号：TM53[电气工程—电器]