超声联合RGDS靶向微泡携尿激酶在体溶栓后血管假性血友病因子和组织因子表达的实验研究  

The expression of von willebrand factor （vWF） and tissue factor （TF） in ultrasound combined with urokinase and RGDS-targeted microbubbles for recanalization of occluded rabbit femoral artery

作　　者：关丽娜[1] 王春梅[1] 吐尔逊娜依·阿迪[1] 穆玉明[1] 

机构地区：[1]新疆医科大学第一附属医院心脏超声诊断科,乌鲁木齐830054

出　　处：《中华超声影像学杂志》2016年第7期620-624,共5页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金（30860267）

摘　　要：目的探讨低频超声（US）联合精氨酸一甘氨酸一天冬氨酸一丝氨酸（RGDS）靶向微泡携尿激酶（UK）在体溶解兔股动脉血栓后血管假性血友病因子（VWF）和组织因子（TF）的表达情况。方法42只新西兰大白兔单侧股动脉制作富含血小板的混合性血栓，随机分为7组，每组6只：①单纯超声照射（US）组；②超声照射＋非靶向微泡造影剂（US＋M）组；③单纯尿激酶静脉注射（UK）组；④超声照射＋非靶向微泡造影剂＋尿激酶静脉注射（US＋M＋UK）组；⑤超声照射＋RGDS微泡造影剂（US＋R）组；⑥RGDS微泡造影剂＋尿激酶静脉注射（R＋UK）组；⑦超声照射＋RGDS微泡造影剂＋尿激酶（US＋R＋UK）组。监测血流量对溶栓效果进行在体评价；120min后，处死动物后行vWF和TF染色。结果血流量显示，US＋R＋UK组实现了完全再通，R＋UK组实现部分再通，其余组均未实现再通。vWF和TF在US、US＋M、US＋R、R＋uK组均为阳性，Us＋M＋UK、UK、US＋R＋UK组为阴性。结论低频超声及靶向微泡携尿激酶的联合作用可抑制vWF和TF的表达，进而促进溶栓，但血液循环的再灌注会影响vWF和TF表达。Objective To explore the express of von willebrand factor（vWF） and tissue factor（TF） in ultrasound combined with urokinase and RGDS-targeted microbubbles for recanalization of occluded rabbit femoral artery. Methods A total of 42 rabbits with platelet-rieh thrombi in the femoral artery were randomized into 7 treatment groups （ n = 6 in each group） ：①ultrasound alone （US） group;②ultrasound, non targeted microbubbles （ US ＋ M） group ; ③ urokinase alone （UK） group ; ④ ultrasound, non-targeted microbubble and urokinase （ US ＋ M ＋ UK） group ; ⑤ ultrasound, platelet-targeted microbubble （ US ＋ R） group; ⑥ platelet-targeted microbubble, urokinase （R ＋ UK） group; ⑦ ultrasound, platelet-targeted mierobubble and urokinase （US ＋ R ＋ UK） group. A total of 6 ml of infusion liquor of urokinase, RGDS and microbubbles （SonoVue） were mixed by the direct conjugation method, infusion via vein within 20 min. Ultrasound was conducted to lyse the clot for 30 min. The recanalization and the velocity tracing change of blood flow in thrombolytic process and the express of vWF and TF were evaluated at 12 min post treatment. Results For US, UK, US ＋ M, US ＋ R and US ＋ M ＋ UK groups, recanalization were failed, The R ＋ UK and US ＋ R ＋ UK groups were partly recanalizated or completed recanalized （ P 〈0. 001 ）. vWF and TF were positive in US, US＋M,US＋R and R＋UK group, vWFand TFwere negative in US＋M＋ UK,UK and US ＋ R ＋ UK groups. Conclusions The combined effects of low frequency ultrasound and targeted microbubble combined with urokinase can inhibit the expression of vWF and TF, and then promote the thrombolysis, however it can affect the expression of vWF and TF after reperfusion.

关 键 词：超声检查 靶向微泡 血栓溶解疗法 

分 类 号：R445.1[医药卫生—影像医学与核医学]