加味竹叶石膏汤的抗炎作用及机制分析  

Anti-inflammatory Effects and Mechanism of Modified Zhuye Shigao Decoction

作　　者：陈艳林[1] 吴生元[2] 徐翔峰 吴晶金[1] 彭江云[1] 万春平[1] 杨会军[1] 

机构地区：[1]云南中医学院第一附属医院,昆明650021 [2]云南中医学院,昆明650500 [3]舟山医院,浙江舟山316000

出　　处：《中国实验方剂学杂志》2016年第15期117-121,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960476);云南省自然科学基金项目(2011FZ262);云南省卫生科技内设研究机构项目(2011WS0024)

摘　　要：目的:研究加味竹叶石膏汤抗炎作用,揭示其作用的机制,阐明吴氏学派治疗痛风(湿热蕴结)临床中药优效的科学基础。方法:C57BL/6小鼠120只,每50只分别采用尿酸钠结晶复制C57BL/6小鼠腹膜炎模型和痛风性关节炎模型;2组模型均随机分组为加味竹叶石膏汤高、中、低剂量组(11.48,5.74,2.87 g·kg-1),阳性药秋水仙碱组(0.65 mg·kg-1),模型组,另设正常组,每组10只。经药物干预后,采集血清,酶联免疫吸附法(ELISA)检测2组模型组内不同组别血清白细胞介素-1β(IL-1β),半胱氨酸天冬氨酸酶(Caspase-1)水平,观察对小鼠急性炎症反应的影响。痛风性关节炎模型造模后6 h,进行关节炎症评分;48 h后取关节组织(滑膜、软骨与骨),石蜡切片苏木素-伊红(HE)染色分析小鼠关节炎病理损伤程度。结果:与正常组比较,模型组小鼠血清中IL-1β,Caspase-1水平明显升高(P<0.01),模型组炎症浸润较明显,关节炎症状较明显;与模型组比较,加味竹叶石膏汤高、中、低剂量组均明显降低小鼠血清中IL-1β,Caspase-1水平(P<0.05,P<0.01);同时明显减轻炎症细胞浸润,改善小鼠关节炎症状。结论:加味竹叶石膏汤具有一定的抗炎作用,其作用机制与抑制炎症细胞因子IL-1β,Caspase-1产生有关。Objective： To investigate the anti-inflammatory effect of modified Zhuye Shigao decoction and its relevant mechanisms,in order to clarify the scientific basis for superior efficiency of traditional Chinese medicines in the clinical treatment of GA（ damp heat accumulation） according to Wu School＇ s therapy for gout.Method： Totally 120 C57 BL /6 mice were selected. The models of peritonitis and acute gouty arthritis were induced with monosodium urate（ MSU） crystals,with 50 C57 BL /6 mice for each model. Both the mice models were randomly divided into different groups,namely the model group,the positive control group（ colchicine,0. 65mg·kg- 1）,the modified Zhuye Shigao decoction group（ 11. 48,5. 74,2. 87 g·kg- 1）,and the normal control group,with 10 mice in each group. After the intervention with different medicines,serums of both inflammation models were collected to detect the inflammatory mediators interleukin-1（ IL-1β） and Caspase-1 with enzymelinked immunosorbent assay,and observe the effect of medicines for the acute inflammation response. The joint inflammatory reaction was induced by injecting MSU crystal suspension inside the ankle joint of the right hind limb.The arthritis was scored at 6 h after modeling; joint tissues（ synovial membrane,cartilage and bone） were collected at 48 h to prepare paraffin sections,and analyze the pathological damage degree of acute gouty arthritis in mice with histological examination（ HE） assay. Result： Serum IL-1β and Caspase-1 of the model group were significantly higher than the normal group（ P 〈0. 01）,with more obvious inflammatory infiltration and arthritis symptom in the model group; serum IL-1β and Caspase-1 of mice in the modified Zhuye Shigao decoction groups were lower than the model group（ P 〈0. 05,P〈 0. 01）,with remarkable alleviation in inflammatory cell infiltration and symptoms of arthritis in mice. Conclusion： Modified Zhuye Shigao decoction has an antiinflammatory effect,whose mechanism may be related to

关 键 词：加味竹叶石膏汤 腹膜炎模型 痛风性关节炎模型 抗炎作用 白细胞介素-1Β 半胱氨酸天冬氨酸酶-1 

分 类 号：R542.42[医药卫生—心血管疾病]