芹菜素对大鼠骨肉瘤细胞凋亡及相关蛋白Bax,Bcl-2,Caspase-3,Caspase-9,细胞色素C的影响  

Effect of Apigenin on Rat Osteosarcoma Spoptosis and Bax,Bcl-2,Caspase-3,Caspase-9,and Cyt-C proteins

作　　者：程新燕[1] 符翠莉[1] 

机构地区：[1]广西壮族自治区人民医院,南宁530021

出　　处：《中国实验方剂学杂志》2016年第15期139-142,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西高校科学技术研究项目(LX2014085)

摘　　要：目的:探讨芹菜素对大鼠骨肉瘤细胞凋亡及相关蛋白B细胞淋巴瘤/白血病-2(Bcl-2),Bcl-2相关X蛋白(Bax),半胱氨酸天冬氨酸蛋白酶-3(Caspase-3),Caspase-9及细胞色素C的影响。方法:SD大鼠50只,建立大鼠骨肉瘤模型,随机分为模型组,顺氯氨铂组(10 mg·kg-1),芹菜素低、中、高剂量组(1,2,4 mg·kg-1),每组10只,ig给药,连续给药30 d。模型组ig给等体积的生理盐水。末次给药24 h后,切取骨肉瘤组织,称质量。TUNEL法原位标记凋亡的骨肉瘤细胞;免疫组化法检测骨肉瘤组织中Bax,Bcl-2的蛋白表达;蛋白质免疫印迹(Western blot)法检测骨肉瘤组织中Caspase-3,Caspase-9,细胞色素C的蛋白含量。结果:与模型组比较,芹菜素低、中、高剂量组的骨肉瘤的质量降低,细胞凋亡数量明显增加,明显上调Bax,Caspase-3,Caspase-9,细胞色素C蛋白的表达,下调Bcl-2蛋白的表达(P<0.05)。结论:芹菜素可通过上调Bax,Caspase-3,Caspase-9,细胞色素C的蛋白表达,下调Bcl-2的蛋白表达,抑制骨肉瘤细胞的增殖,诱导骨肉瘤细胞凋亡,从而对骨肉瘤产生治疗和抑制作用。Objective： To study the effect of apigenin in treatment of rats osteosarcoma apoptosis and Bcell lymphoma 2（ Bcl-2）,Bcl-2 assaciated X protein（ Bax）,Caspase-3,Caspase-9 and nuclear factor-κB（ NF-κB） protein expressions. Method： Totally 50 rats were randomly divided into model group,cisplatin group（ 10mg·kg- 1）,apigenin groups（ 1,2,4 mg·kg- 1）,with 10 rats in each group. The rat osteosarcoma model was made by operation. All groups were given drups for 30 days. Oteosarcoma was collected and weighed 24 h later after the last administration. TUNEL assay was adopted for in situ labeling of apoptosis of osteosarcoma cells.Immunohistochemistry was used to detect expressions of Bax,Bcl-2 in osteosarcoma. Caspase-3,Caspase-9 and cytochrome C（ Cyt-C） expressions in osteosarcoma were tested using Western blot assay. Result： Compared with model group,apigenin-treated groups showed decrease in osteosarcoma weight,significant increase in the number of apoptotic cells,obvious up-regulation in Bax,Caspase-3,Caspase-9 and Cyt-C protein,and down-regulation in the expression of Bcl-2. Conclusion： Apigenin can increase Bax,Caspase-3,Caspase-9,the expression of Cyt-C,down-regulate the expression of Bcl-2,inhibit the proliferation of osteosarcoma cells,and induce apoptosis in osteosarcoma cells,so as to show a therapeutic and inhibitive effect on osteosarcoma.

关 键 词：芹菜素 骨肉瘤 细胞凋亡 B细胞淋巴瘤/白血病-2 Bcl-2相关X蛋白 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R285.5[医药卫生—中药学]