对氯苯丙氨酸抑制野百合碱诱导的大鼠肺动脉和右心室重构  

PCPA inhibits monocrotaline-induced remodeling of pulmonary artery and right ventricle in rats

作　　者：白洋[1] 王韵[1] 李中夏 高明奇[1] 廉国超 王怀良[1] 

机构地区：[1]中国医科大学临床药理教研室,沈阳110122 [2]沈阳医学院附属中心医院,沈阳110024

出　　处：《解剖科学进展》2016年第4期372-374,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30572194,30973533)

摘　　要：目的研究对氯苯丙氨酸(4-Chloro-DL-phenylalanine,PCPA)对野百合碱(monocrotaline,MCT)诱导的大鼠肺动脉和右心室重构的影响。方法建立MCT诱导的大鼠肺动脉高压(pulmonary arterial hypertension,PAH)模型,腹腔注射给予不同剂量PCPA。检测肺动脉压力、右心指数等血流动力学指标。HE染色观察肺动脉及心肌形态学的改变。Western Blot方法检测右心室基质金属蛋白酶13(matrix metalloproteinases 13,MMP-13)的表达。结果 MCT诱导的大鼠肺动脉压力、右心指数、肺动脉血管壁厚度、心肌细胞的损伤及右心室中MMP-13蛋白表达水平比对照组明显增加,而PCPA则剂量依赖性的降低以上各个指标。结论 PCPA抑制MCT诱导的大鼠肺动脉和右心室的重构可能与降低MMP-13蛋白表达相关。Objective To study lhe effect of 4-Chloro-DL-phenylalanine（PCPA） on monocrolaline（MCT）- induced remodeling of pulmonary arlery and right ventricle in rats. Methods Pulmonaryterial hypertension models were induced by monoertaline and PCPA （intraperitoneal injection of PCPA）. The pulmonary arterial pressure and righl ventricle hypertrophy index were measured, the change of puhnonary artery and myocardial morphology was observed by H E straining, the expression of matrix metalloproteinases-13（MMP-13） in right ventricle was detected by Western blot. Results The, pulmonary artely pressure, right ventricle hyperlmphy index, pulmonary vascular wall thickness, the damage of myocardial cells and expmssinn level of MMP-13 prolein in righl ventricle were increased significantly compared to the normal control, bul significanlly deere＆sed hy PCPA with dose-dependent manner. Conclusion The inhibition of the pulmonary arterial remodeling and righl venlrieular hyperlmphy by PCPA might be related to the decrease of MMP-13 protein.

关 键 词：对氯苯丙氨酸 野百合碱 肺动脉高压 重构 大鼠 

分 类 号：R965[医药卫生—药理学]