糖尿病大鼠肺组织PI3K/Akt表达的变化及意义  

Changes and significance of PI3K/Akt expression in lung tissue of diabetic rats

作　　者：周德鹏 梁英杰 曹东华[2] 徐华 

机构地区：[1]辽河油田总医院检验科,辽宁盘锦124010 [2]中国人民解放军第二0二医院检验科,辽宁沈阳110003

出　　处：《解剖科学进展》2016年第4期426-428,共3页Progress of Anatomical Sciences

基　　金：辽宁省教育厅科研基金(L2010687)

摘　　要：目的研究糖尿病大鼠肺组织磷脂酰肌醇3激酶(PI3K)/丝氨酸-苏氨酸激酶(Akt)表达的变化及意义。方法 40只大鼠随机分为对照组(10)和糖尿病组(30),通过高糖、高脂饮食加腹腔注射小剂量链脲佐菌素(STZ)的方法建立2型糖尿病大鼠模型。糖尿病模型建立后,检测血糖、血脂的改变;采用免疫组织化学方法和Western blot方法检测各组大鼠肺组织中PI3K、Akt和p-Akt表达的变化情况。结果与对照组比较,糖尿病模型组大鼠血糖、血脂值明显增高(<0.01)。免疫组织化学和Western blot结果显示,糖尿病模型组大鼠模肺组织PI3K、Akt和pAkt的表达量均显著高于对照组。结论 PI3K/Akt信号通路可能参了与糖尿病肺组织病变的发生发展。Objective To study the changes and significance of PI3FUAkt expression in hmg tissue of diabetic rats. Methods 60 rats were divided into control group（10） and diabetes mellitus group （30）, and the diabetic ral models were established by giving high glucose, high fat diet combined wilh peritoneal injection with low-dose streptozotnein （STZ）. The changes of blood glucose and blood lipids were measured after the establishment of the model of diabetes, the expressions of PI3K, Akt and p-Akt were observed by innnunohistoehemistry and Western blot in lungs of rats in all groups. Results Compared with the conrail group, serum glucose and lipid values were significantly higher in rats of diabetes mellitus group, the expression levels of PI3K and Akt and p-Akt ptoteins were incleased significantly in lung tissue of diabetic rats. Conclusion PI3FUAkt signal pathway might be involved in hmg tissue pathological changes of dibtetic rats.

关 键 词：糖尿病 肺 磷脂酰肌醇3激酶 丝氨酸-苏氨酸激酶 大鼠 

分 类 号：R587.1[医药卫生—内分泌]