UPLC-MS/MS法同时测定安喘舒丸中腺苷和黄芪甲苷含量  

Simultaneous Determination of Astragaloside Ⅳ and Adenosine in Anchuanshu Pill by UPLC-MS/MS

作　　者：华云鹏 雍太萍 宗宁峰 许桂丽[1] 陆瑜[1] 

机构地区：[1]解放军第454医院药学部,江苏南京210002

出　　处：《中国现代中药》2016年第8期1055-1057,共3页Modern Chinese Medicine

摘　　要：目的:建立同时测定安喘舒丸中腺苷、黄芪甲苷含量的方法。方法:采用液质联用技术,以甲醇-0.1%甲酸溶液为流动相等度洗脱,以Agilent ZORBAX SB-C_(18)色谱柱为分析柱,通过电喷雾离子源,选择正离子模式多反应监测方式检测。结果:腺苷、黄芪甲苷的线性范围分别为10~320 ng·m L^(-1)(r=0.996 8)、5~160 ng·m L^(-1)(r=0.995 5),加样回收率在96.17%~99.48%,RSD均低于1.14%。结论:该法专属性强、快速灵敏,可用于安喘舒丸中腺苷、黄芪甲苷的含量测定。Objective： To develop a UPLC-MS/MS method for the determination of adenosine and astragaloside IV in Anchuanshu Pill. Methods： Isocratic elution was carried out with mobile phase consisting of methanol - 0. 1% formic acid. The separation was performed on an Agilent ZORBAX SB-C18 column, and the mass spectrometer was operated in the positive ionization electrospray （ESI） mode using multiple monitoring （MRM） for analysis of two components. Results： Adenosine and astragaloside IV were all determined exactly, the linear ranges were 10-320 ng. mL -1 （ r = 0. 996 8 ）, 5-160 ng·mL^-1 （ r = 0. 995 5 ） , respectively. The recoveries of two analytes ranged from 96. 17%-99. 48% and the relative standard deviations were all below 1.14%. Conclusion： A sensitive, accuracy and suitable UPLC-MS/MS method has been developed, and the method could be applied for the determination of adenosine and astragaloside IV in Anchuanshu Pill.

关 键 词：安喘舒丸 腺苷 黄芪甲苷 超高效液相色谱-串联质谱法 

分 类 号：R286.0[医药卫生—中药学]