基于SCoT与IRAP标记的金枣柿实生后代遗传变异分析及其生殖特性  

Study on the hereditary variation of Jinzaoshi persimmon(Diospyros sp.) seedling progeny and the reproduction characteristics analyses based on SCoT and IRAP markers

作　　者：唐冬兰 胡燕[1] 龚榜初[3] 张青林[1] 罗正荣[1] 

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070 [2]江苏丘陵地区南京农业科学研究所,南京223002 [3]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《果树学报》2016年第8期934-942,共9页Journal of Fruit Science

基　　金：公益性行业(农业)科研专项"现代柿产业关键技术研究与试验示范"(201203047);国家科技基础性工作专项(2012FY110100)

摘　　要：【目的】金枣柿是浙江重要经济果树之一,其果实有无核与有核之分,在雄花未见的情况下,其后代遗传变异情况及生殖特性有待探讨。【方法】以金枣柿母本一的15个子代及母本二的16个子代为研究群体,以来自浙江不同地区的12个金枣柿单株及柿(Diospyros kaki)、君迁子(D.lotus)、山柿(D.japonica)为对照;采用SCo T和IRAP分子标记技术,分析金枣柿实生后代的遗传变异情况,并以此推测其种子来源。【结果】利用筛选出的21条SCoT引物及20条IRAP引物对供试材料基因组DNA的扩增结果表明,2种标记共扩增出473条清晰、可重复谱带,其中多态性谱带454条;2种标记的多态性比例分别为93.56%和97.50%。2个子代群体均产生了遗传分离。群体1通过SCo T和IRAP标记得到的DICE相似系数变异分别为0.637~1.000和0.927~1.000,群体2利用这2种标记得到的DICE相似系数变异分别为0.987~1.000和0.981~1.000。【结论】金枣柿的实生群体中均存在一定程度的遗传变异,其中群体1的DICE变异系数虽低于群体2,但其变异主要集中于个别单株,群体2单株的变异类型比群体1更丰富。金枣柿的种子主要由有性杂交产生,其花粉供体很可能为同种的雄花,不排除其具有兼性无融合生殖的能力。[Objective]Jinzaoshi persimmon （Diospyros sp.） is one of the important economic fruit trees in Zhejiang province. It is generally considered a new species of Diospyros. Its fruits can be seedless or seed bearing in different producing areas. However, its staminate flowers remain undetected and its reproduc- tion characteristics remain mistery. Some plants producing seeds stably were found in Qingyuan county of Zhejiang province. Seedling populations were used for studying the hereditary variations and reproduction characteristics by using SCoT and IRAP markers. The reproduction character of Jinzaoshi was also ana- lyzed based on the hereditary variation of these seedlings. [ Methods ] Two batches of seeds from two Jinza- oshi persimmon plants were collected in Qingyuan county, Zhejiang province in December of 2013. Then, the seeds were sowed in Persimmon Repository of Subtropical Forestry Research Institute, Chinese Acade- my of Forestry. In 2014, fifteen individuals from QY1 （population 1） and 16 individuals from QY2 （popula- tion 2） were randomly selected from the two seedling populations. Twelve individuals of Jinzaoshi persim-mon from other locations and each plant of three Diospyros species （D. kaki, D. lotus and D. japonica） were used as control. By using SCoT and IRAP markers, the hereditary variations of these individuals were eval- uated. Multiplex ratio, polymorphic information content and marker index were calculated. The calcula- tion of DICE similarity coefficient and clustering analysis were carried out by NTSYSpc version2.10e soft- ware. [ Results ] In 36 selected SCoT primers and 26 IRAP primers, 21 SCoT primers and 20 IRAP prim- ers performed well in the tested materials, respectively. The results abtained by these primers showed that the polymorphism level detected by IRAP markers was slightly higher than that by SCoT markers. A total of 473 clear and reproducible bands were obtained, of which 454 were polymorphic and the polymorphism frequencies were 93.56% （SCOT） and 97.50%

关 键 词：金枣柿 SCoT IRAP 种子形成 生殖特性 

分 类 号：S665.2[农业科学—果树学]