基于电感耦合等离子体质谱的单细胞分析  

Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis

作　　者：史俊稳[1,2] 张欣颖[2,3] 李亮[3] 丰伟悦[2] 王海芳[1] 王萌[2] 

机构地区：[1]上海大学环境与化学工程学院,上海200444 [2]中国科学院高能物理研究所纳米生物效应与安全性重点实验室,北京100049 [3]武汉工程大学材料科学与工程学院,武汉430205

出　　处：《生物化学与生物物理进展》2016年第8期739-746,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(21175136;U143220027)~~

摘　　要：单细胞分析可以获得细胞在微环境中准确的个体信息,对于研究细胞的信号传导、生理病理和疾病的早期诊断等具有十分重要的意义.近年来,基于电感耦合等离子体质谱(ICP-MS)的单细胞分析方法开始得到越来越多的应用.本文综述了基于ICP-MS的单细胞分析方法及其在免疫分析、疾病诊断、药物筛选、纳米分析等方面的部分应用,并对基于ICP-MS的单细胞分析方法做出总结和展望.Previous studies generally focused on the cell colony and the obtained information was commonly the average of many cell individuals.However,each cell has a different behavior,which is known as cell heterogeneity.Single cell analysis can accurately obtain valuable information of each cell in the microenvironment and thus there is an urgent need for single cell analysis.Many methods have been successfully employed to single cell analysis,such as flow cytometry,fluorescence microscopy,capillary electrophoresis and microfluidic chips.In these methods,single cells are usually stained by a fluorescent label（e.g.fluorescein,quantum dots,green fluorescent protein,etc.） and then detected via the fluorescent signal.However,simultaneous analysis of multiple parameters in a single cell is always challenging because of the overlap in fluorescent spectrum.In addition,the linear range of the fluorescence method is relatively narrow,making difficulty for accurate quantification,especially when comparing signals with considerable difference.To meet these challenges,a new method based on inductively coupled plasma mass spectrometry（ICP-MS） has emerged for single cell analysis.Intracellular elements can be determined directly by ICP-MS at a single cell level.In combination with labeling techniques（e.g.element labeling of an antibody）,biomolecules in single cells can also be determined via elements analysis by ICP-MS.This paper summaries both the ICP-MS-based methodology and selected applications in immunoassay,disease detection,drug screen,and nanoanalysis at a single cell level.A prospective of this method and its applications is also discussed.

关 键 词：电感耦合等离子体质谱 单细胞分析 金属标签 质谱细胞仪 激光烧蚀 

分 类 号：O657.63[理学—分析化学]