穴位注射型电针治疗仪对炎性痛大鼠消炎镇痛作用的研究  

Anti-inflammatory and analgesia effects of electroacupuncture device of point injection on rats of inflammatory pain

作　　者：范月媛 黄国付[1] 高芳[2] 武彩花 苑小翠 李洪萍[2] 潘晓莉[3] 陈威 曹炀 邢鲁栋 李熳[2] 

机构地区：[1]湖北中医药大学附属中西医结合医院,武汉430022 [2]华中科技大学同济医学院神经生物学系,湖北武汉430030 [3]华中科技大学同济医学院附属协和医院 [4]武汉市康本龙医疗器械有限公司 [5]苏州医疗用品厂有限公司

出　　处：《中国针灸》2016年第8期845-850,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81173324;81473768;武汉市科技局项目:201306060201028;中央高校基本业务费资助:2172012YLQX006

摘　　要：目的：研究穴位注射型电针治疗仪对炎性痛大鼠消炎镇痛作用的机制。方法：SD雄性大鼠48只,随机分为对照组、模型组、电针＋穴位注射组（EA＋PI组）、穴位注射型电针治疗仪组（EAPI组）、电针组（EA组）、穴位注射组（PI组）,每组8只。对照组大鼠在左后肢足背皮下注射50μL溶媒液体石蜡油;余组在同一部位注射50μL完全弗氏佐剂（complete freund’s adjuvant,CFA）诱导炎性痛模型。EA＋PI组、EAPI组、EA组、PI组足背注射CFA造模后隔日（第2、4、6d）分别给予电针联合穴位注射、穴位注射型电针治疗仪、电针、穴位注射治疗,每次持续30min。观察各组大鼠造模前和造模后1-6d机械痛阈值、热痛阈值及足部肿胀程度的变化,并采用Western blotting法检测干预结束后各组大鼠炎性局部皮肤组织中IL-1β蛋白的表达。结果：炎性痛模型建立后,与对照组比较,余组大鼠机械痛阈值和热痛阈值均降低（均P〈0.05）,而足背肿胀程度升高（均P〈0.05）;治疗后,EAPI组大鼠的机械痛阈、热痛阈值较模型组、EA＋PI组、EA组和PI组显著升高（均P〈0.05）,而足背肿胀程度则降低（均P〈0.05）。模型组IL-1β蛋白的表达较对照组增加（P〈0.05）,经治疗后,EAPI组IL-1β蛋白表达低于模型组、EA组和PI组（均P〈0.05）,而与EA＋PI组比较差异无统计学意义（P〉0.05）。结论：穴位注射型电针治疗仪治疗炎性痛的疗效优于电针结合穴位注射疗法和单纯电针或单纯穴位注射疗法。Objective To explore the anti-inflammatory and analgesia mechanism of electroacupuncture（EA）device of point injection（PI）on rats of inflammatory pain.Methods 48 Sprague Dawley（SD）rats were randomly assigned into a control group,a model group,an EA＋PI group,an EA device of PI（EAPI）group,an EA group and a PI group,eight rats in each one.The rats in the control group were subcutaneously injected with 50μL of liquid paraffin oil solvent into the dorsum of left hindpaw,while rats in the remaining groups were treated with 50μL of complete freund＇s adjuvant（CFA）at identical location to induce the model of inflammatory pain.After model establishment,the rats in the EA＋PI group,EAPI group,EA group and PI group were treated with EA＋PI,EA device of PI,EA and PI,respectively,once every other day（the 2nd day,4th day and 6th day）.Each treatment was given for 30 min.The mechanical withdrawal threshold,thermal withdrawal threshold and foot swelling before and 1dto 6dafter model establishment were observed;the western blotting method was applied to measure IL-1βexpression in inflammatory tissue of skin.Results After model establishment,compared with the control group,the mechanical withdrawal threshold and thermal withdrawal threshold were reduced（all P〈0.05）and the foot swelling was increased in the rest groups（all P〈0.05）.After treatment,the mechanical withdrawal threshold and thermal withdrawal threshold in the EAPI group were significantly increased compared with those in the EA＋PI group,EA group and PI group（all P〈0.05）,but the foot swelling was reduced（all P〈0.05）.The IL-1βexpression in the model group was higher than that in the control group（P〈0.05）;after treatment,the IL-1βexpression in the EAPI group was lower than that in the model group,EA group and PI group（all P〈0.05）,but no significantly different from that in the EA＋PI group（P〉0.05）.Conclusion The efficacy of EA device of PI on inflammatory pain is superior to EA combined with PI,EA

关 键 词：炎性痛 电针 穴位注射 IL-1Β 针刺镇痛 

分 类 号：R246.2[医药卫生—针灸推拿学]