氨磷汀对人巨核细胞白血病Dami细胞增殖及分化的影响  

Effect of amifostine on proliferation and differentiation of human megakaryocyte Dami cells

作　　者：汪海涛[1,2,3] 杨波[1] 卢学春[1] 胡博[2,4] 杨红旗[1] 罗龙龙[2] 林洁[1] 李素霞[1] 范辉[1] 乔春霞[2] 王巍[2] 郎晓玲[2] 耿晶[2] 黎燕[2] 吴晓雄[3] 吕明[2] 朱宏丽[1] 

机构地区：[1]中国人民解放军总医院老年血液科,北京100853 [2]军事医学科学院基础医学研究所免疫学研究室,北京100850 [3]中国人民解放军总医院第一附属医院血液科,北京100048 [4]北京大学肿瘤医院胸外科,北京100142

出　　处：《中国药理学与毒理学杂志》2016年第7期723-727,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金项目(81273597);解放军总医院科技创新苗圃基金(15KMM28);全军保健基金(13BJ247)~~

摘　　要：目的探讨氨磷汀(依硫磷酸,Amf)对人巨核细胞白血病Dami细胞分化的影响。方法 Amf0.01~5.0 mmol·L^(-1)分别处理Dami细胞12 d,每天光镜下观察细胞形态,计数Dami细胞数量,绘制增殖曲线;第12天,计数直径>20μm细胞数量,透射电镜观察Dami细胞血小板分界膜系统的形成,流式细胞仪检测细胞DNA倍体化及细胞表面抗原CD33,CD34和CD41a的变化。结果 Amf 0.1~1.0 mmol·L^(-1)促进Dami细胞分化;Amf>1.0 mmol·L^(-1)时抑制Dami细胞增殖,Amf 1.0 mmol·L^(-1)作用12 d后光镜下观察到细胞体积增大,直径>20μm细胞比例增加24.6%(P<0.01);透射电镜观察可见细胞出现血小板分界膜系统;流式细胞仪发现,髓系细胞分化抗原CD33表达减少13.6%;巨核细胞相关分化抗原CD41a表达增加11.9%(P<0.05);DNA倍体分析发现,实验组4N和8N细胞分别增至31.56%和8.83%(P<0.01),并出现16N的超多倍体细胞(3.43%)。结论 Amf>1.0 mmol·L^(-1)时抑制Dami细胞增殖,0.1~1.0 mmol·L^(-1)时诱导Dami细胞向成熟巨核细胞分化。OBJECTIVE To investigate the effect of amifostine（Amf）on the differentiation of human megakaryocyte cell line-Dami. METHODS Dami cells were treated with Amf 0.01-5.0 mmol·L-（-1）for 12 d.Dami cells were counted every day for the growth curve：only cells with a diameter 20 μm. The platelet demarcation membrane system was observed by transmission electron microscopy. The expression of CD33,CD34,CD41 a and DNA ploidy was detected by flow cytometry. RESULTS Amf 0.1-1.0 mmol·L-（-1）promoted the differentiation of Dami cel s,but inhibited their proliferation at a concentration 1.0 mmol·L-（-1）.When these cells were treated with Amf 1.0 mmol·L-（-1）for 12 d,the platelet demarcation membrane system was observed,the percentage of cells with a diameter 20 μm was increased by 24.6%（P〈0.01）,the expression of CD41 a was increased by 11.9%,while the expression of CD33 was decreased by 13.6%（P〈0.05）. Polyploidy cells（16N）were observed,and 4N,8N and 16 N cells were increased to31.56%,8.83% and 3.43%,respectively（P〈0.05）. CONCLUSION Amf 0.1-1.0 mmol·L-（-1）can promote the differentiation of Dami cells,but inhibit their proliferation at a high concentration（1.0 mmol·L-（-1））.

关 键 词：氨磷汀 Dami细胞 细胞分化 CD41a DNA倍体化 

分 类 号：R96[医药卫生—药理学]