转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定  

Determination of Content and Entrapment Efficiency of Transferrin Modified Vincristine-Tetrandrine Liposomes

作　　者：李学涛[1] 唐炜 姜英[1] 程岚[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]临沂市食品药品检验检测中心,山东临沂276001

出　　处：《中国药房》2016年第22期3034-3036,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81541081);辽宁省科学技术计划项目(No.2014020046);辽宁省教育厅重点实验室基础研究项目(No.LZ2015053)

摘　　要：目的：建立转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及脂质体包封率的测定方法。方法：采用高效液相色谱法测定长春新碱含量,色谱柱为依利特C_（18）,流动相为甲醇-15%三乙胺水溶液（70∶30）,流速为1.0 ml/min,检测波长为297 nm,柱温为30℃,进样量为20μl;采用葡聚糖凝胶柱色谱法分离脂质体与游离药物,计算包封率。结果：长春新碱检测质量浓度为160-1 600μg/ml（r=0.999 8,n=5）,平均加样回收率为99.20%（RSD=0.26%,n=9）,精密度RSD为0.070%（n=5）。脂质体中长春新碱的平均含量为0.790 mg/ml（RSD=0.15%,n=3）,平均包封率为85.94%（RSD=2.08%,n=3）。结论：该方法准确可靠、简单快速,可用于转铁蛋白修饰长春新碱-粉防己碱脂质体中长春新碱的含量及包封率测定。OBJECTIVE：To establish the method for the determination of main components content and entrapment efficiency of transferrin modified vincristine-tetrandrine liposomes. METHODS：HPLC method was adopted to determine the content of vincristine. The determination was performed on ELITE C_（18） column with mobile phase consisted of methanol-15% triethylamine（70 ∶30）at a flow rate of 1.0 ml/min. The determination wavelength was set at 297 nm and column temperature was 30 ℃. The sample size was 20 μl. Free drug was isolated from liposomes by dextran gel column chromatography,and entrapment efficiency was determined. RESULTS：The linear range of vincristine was 160-1 600 μ g/ml（r=0.999 8,n=5） with average recovery of 99.20%（RSD=0.26%,n=9）. RSD of precision test was 0.070%（n=5）. The average content of vincristine in liposomes was 0.790mg/ml（RSD=0.15%,n=3）,and the average entrapment efficiency was 85.94%（RSD=2.08%,n=3）. CONCLUSIONS：The method is accurate,reliable,simple and rapid,and can be used for the determination of the content and entrapment efficiency of vincristine in transferrin modified vincristine-tetrandrine liposomes.

关 键 词：长春新碱 粉防己碱 转铁蛋白 脂质体 含量测定 包封率 高效液相色谱法 

分 类 号：R979.1[医药卫生—药品] R927[医药卫生—药学]