Man-PEI_(25k)纳米复合物在微针作用下的透皮性能研究  

Study on the Percutaneous Properties of Man-PEI_(25k) Nanocomplex under the Treatment of Microneedles

作　　者：许娇娇[1,2] 杨云旭[1] 徐蓓华[1] 胡英[1] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]温州医科大学药学院,浙江温州325000

出　　处：《中国药房》2016年第22期3062-3065,共4页China Pharmacy

基　　金：浙江省大学生科技创新活动计划暨新苗人才计划项目(No.2013R433003);宁波市第二批科技项目(No.2015C110027);宁波市科技创新团队项目

摘　　要：目的:研究高分子质量聚乙烯亚胺(PEI_(25k))的甘露糖衍生物(Man-PEI_(25k))的纳米复合物在微针作用下的透皮性能。方法:以荧光染料水溶性羧基CdSe/ZnS量子点(QD)为模型药物,与PEI_(25k)、Man-PEI_(25k)溶液通过静电吸附形成PEI_(25k)/QD和不同嫁接率(1:3、1:6)的Man-PEI_(25k)/QD纳米复合物,同时以游离的QD作为对照,通过共聚焦显微镜观察微针作用下QD在皮肤活性表皮层和真皮层中的分布情况;用荧光分光光度计测定微针作用下PEI_(25k)/QD和Man-PEI_(25k)/QD纳米复合物在皮肤活性表皮层和真皮层中QD的累积滞留量。结果:微针作用于在体皮肤,Man-PEI_(25k)/QD纳米复合物在活性表皮层和真皮层的量明显高于PEI_(25k)/QD纳米复合物;Man-PEI_(25k)/QD(1:6)纳米复合物在活性表皮层和真皮层的量明显高于Man-PEI_(25k)/QD(1:3)纳米复合物。体外透皮试验显示,微针能明显提高纳米复合物在活性表皮层和真皮层中的滞留量;微针作用48 h后,Man-PEI_(25k)/QD纳米复合物在活性表皮层和真皮层的滞留量约比PEI_(25k)/QD增加了2、1.5倍,二者差异有统计学意义(P<0.01)。结论:微针可提高Man-PEI_(25k)/QD纳米复合物在活性表皮层的透皮性能。OBJECTIVE：To study the percutaneous properties of Man-PEI（25k） nanocomplex under the treatment of microneedles.METHODS：Using fluorescent dye water-soluble carboxyl CdSe/ZnS quantum dot（QD） as model drug,Man-PEWQD and Man-PEWQD nanocomplex with different grafting rates（1：3,1：6） were formed through electrostatic adherence with PEI（25k） and Man-PEI（25k）.The distribution of QD in the active epidermal layer and dermis of skin were observed by confocal microscopy after the treatment of microneedles,using free QD as control.The accumulative retention amounts of QD in the active epidermal layer and dermis of skin were determined by fluorescence spectrophotometer after PEWQD and Man-PEWQD nanocomplex treated with microneedles.RESULTS：The amounts of Man-PEWQD nanocomplex in active epidermal layer and dermis were significantly higher than that of PEI（25k）/QD nanocomplex under the treatment of microneedles in vivo;the amounts of Man-PEI（25k）/QD（1：6） nanocomplex in active epidermal layer and dermis of skin were significantly higher than that of Man-PEWQD（1：3） nanocomplex.In in vitro transdermal diffusion experiments,microneedles could increase the retention amounts of nanocomplex in active epidermal layer and dermis of skin significantly.The retention amounts of Man-PEI（25k）/QD nanocomplex in active epidermal layer were increased by2 times of that of PEI（25k）/QD under the treatment of microneedles after 48 h;at the same time,in the dermis that was increased by1.5 times,with statistical significance（P〈0.01）.CONCLUSIONS：Microneedles can improve the percutaneous properties of Man-PEI（25k） nanocomplex in active epidermal layer.

关 键 词：高分子质量聚乙烯亚胺的甘露糖衍生物 纳米复合物 微针 透皮给药 水溶性羧基CdSe/ZnS量子点 

分 类 号：R965[医药卫生—药理学]