黎药除湿祛风汤对鸡Ⅱ型胶原蛋白诱导关节炎模型大鼠的保护作用研究  

Study on Protective Effects of Li Medicine Chushi Qufeng Decoction on Arthritis Model Rats Induced by Chicken Type Ⅱ Collagen

作　　者：张丽[1] 张一云[2] 符洪[2] 王开裕[1] 雷玲芳[1] 王春桃[2] 

机构地区：[1]海南省中医院药学部,海口570203 [2]海南省药物研究所,海口570216

出　　处：《中国药房》2016年第22期3088-3090,共3页China Pharmacy

基　　金：海南省中药现代化专项项目(No.2015ZY18)

摘　　要：目的：研究黎药除湿祛风汤对鸡Ⅱ型胶原蛋白诱导关节炎模型大鼠的保护作用。方法：将60只大鼠随机分为正常组、模型组、阳性组和除湿祛风汤高、中、低剂量组[45.9、22.95、11.48 g（生药）/kg],除正常组外,其余各组大鼠均以鸡Ⅱ型胶原蛋白诱导关节炎模型。建模后,除湿祛风汤各剂量组大鼠ig相应药物,正常组和模型组大鼠ig生理盐水,每天1次,连续给药12 d;阳性组大鼠实验1-3 d ig来氟米特4.5 mg/kg,4-12 d ig来氟米特1.8 mg/kg,每天1次。观察大鼠关节病变程度并进行评分,测定关节肿胀度和血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）和Ⅱ型胶原蛋白抗体水平。结果：与正常组比较,模型组大鼠关节炎指数评分、关节肿胀度及血清中TNF-α、IL-1β、Ⅱ型胶原蛋白抗体水平显著升高（P〈0.01）。与模型组比较,阳性组和除湿祛风汤高剂量组大鼠病理评分显著降低,各给药组大鼠血清中TNF-α、IL-1β、Ⅱ型胶原蛋白抗体水平显著降低（P〈0.05或P〈0.01）。结论：黎药除湿祛风汤对鸡Ⅱ型胶原蛋白诱导模型大鼠具有一定的保护作用。OBJECTIVE：To study the protective effects of Li medicine Chushi qufeng decoction on arthritis model rats. METHODS：60 rats were randomly divided into normal group,model group,positive group and Chushi qufeng decoction high-dose,medium-dose and low-dose groups [45.9,22.95,11.48 g（crude drug）/kg]. Except for normal group,those groups were given chicken type Ⅱ collagen to induce arthritis model. After modeling,normal group and model group were given normal saline intragastrically,once a day,for consecutive 12 d;Chushi qufeng decoction groups were given relevant medicine intragastrically;positive group was given leflunomide 4.5 mg/kg on 1st-3rd day and 1.8 mg/kg on 4th-12 th day. The degree of joint lesion in rats were scored. The degree of joint swelling was determined as well as the serum levels of TNF-α,IL-1 β and type Ⅱ collagen antibody. RESULTS：Compared with normal group,arthritis index,degree of joint swelling,the serum levels of TNF-α,IL-1β and type Ⅱ collagen antibody increased significantly in model group（P〈0.01）. Compared with model group,pathological score of positive group and Chushi qufeng decoction high-dose group decreased significantly,and serum levels of TNF-α,IL-1β and type Ⅱ collagen antibody decreased significantly in treatment groups（P〈0.05 or P〈0.01）. CONCLUSIONS：Li medicine Chushi qufeng decoction has certain protective effect on arthritis model rats induced by chicken type Ⅱ collagen.

关 键 词：黎药 除湿祛风汤 类风湿性关节炎 肿瘤坏死因子α 白细胞介素1Β 大鼠 

分 类 号：R943[医药卫生—药剂学]