己酮可可碱在阿霉素心肌病大鼠中的抗炎症作用  

Anti-inflammatory Effects of Pentoxifylline after Adriamycin-induced Cardiomyopathy in Rats

作　　者：袁晓峰[1] 臧志军[2] 袁群芳[3] 

机构地区：[1]中山大学附属第三医院岭南医院综合ICU,广东广州510630 [2]中山大学附属第三医院不育与性医学科,广东广州510630 [3]中山大学中山医学院人体解剖教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2016年第4期543-547,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：中山大学青年教师培育项目(16ykpy44)

摘　　要：【目的】探讨己酮可可碱在阿霉素心肌病大鼠模型中的作用及机制研究。【方法】6周龄雄性SD大鼠30只,随机分为3组:1生理盐水组;2阿霉素(ADR)组;3阿霉素(ADR)+己酮可可碱(PTX)组。动物药物处理3周后,取大鼠的心脏组织作病理学分析并提取RNA检测炎症因子TNF-α、IL-1β、IL-10的表达情况;同时研磨心肌组织,提取蛋白,ELISA检测TNF-α、IL-1β、IL-10的表达情况。并且采用高频多普勒超声检测评价实验中各组心脏收缩功能。【结果】组织病理学表明PTX能缓解ADR引起的大鼠心肌损伤,ADR+PTX组的心肌炎症细胞浸润程度明显比ADR组减轻。RT-PCR、心肌ELISA检测显示与生理盐水组相比较,ADR组TNF-α、IL-1β表达量增加,而PTX处理组显著降低其炎症因子的表达;与ADR组相比,ADR+PTX组中IL-10表达量呈上升趋势。同样与ADR组相比较,ADR+PTX组的左心射血分数升高、左心室长轴缩短率增加。【结论】PTX对阿霉素引起的心肌纤维化的保护作用可能是通过对IL-10表达调控来实现的。PTX可能成为临床治疗阿霉素心肌病的有效的选择药物。[ Objective]To explore the anti-inflammatory effects of pentoxifylline after adriamyein-induced eardiomyopathy in rats. [Methods] Sprague-Dawley rats （6-week-old）were randomly assigned to saline,ADR [Adriamycin,5 mg/（kg .week）, ADR [Adriamycin, 5 mg/（kg.week）] ＋ PTX [pentoxifylline, 50 mg/（kg.d）] groups. After 3 weeks, heart tissue was harvested from each animal and used for histological analysis. The expression of TNF-α,IL-1β,IL-10 were examined by RT-PCR and ELISA. Meanwhile, the left ventricular ejection fractions （LVEF） and longitudinal fractional shortening （LVLFS） were detected byhigh frequency Doppler ultrasound. [Results] H＆E histological analysis showed that treatment with ~ could alleviate myocardial damage and reduce inflammatory cells infiltration compared to the ADR-treated control rats. The mRNA and protein expression of TNF-α, IL- 1β was significantly decreased in ADR＋PTX group compared with ADR group. In contrast, the IL-10 was up-regulated in the ADR＋ PTX group, and down-regulated in the ADR group. The left ventricular ejection fractions and longitudinal fractional shortening increased in the ADR ＋PTX group than the ADR group. [Conclusion] PTX exerts a protective effect against ADR-induced cardiomyopathy via regulation of inflammatory response TNF-α, IL-1β, L-10. PTX may be a new drugcandidate for the treatment of ADR-induced cardiomyopathy.

关 键 词：心肌病 己酮可可碱 白介素-10 左心射血分数 

分 类 号：R54[医药卫生—心血管疾病]