Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China  

分离自成都大熊猫兽舍空气中的一株金黄杆菌属新种的鉴定（英文）

作　　者：Cai-fang WEN Li-xin XI Shan ZHAO Zhong-xiang HAO Lu LUO Hong LIAO Zhen-rong CHEN Rong SHE Guo-quan HAN San-jie CAO Rui WU Qi-gui YAN Rong HOU 

机构地区：[1]College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China [2]College of Food Science, Sichuan Agricultural University, Yaan 625014, China [3]Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年第8期610-618,共9页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Chengdu Giant Panda Breeding Research Foundation(No.CPF2010-06);the National Key Technology R&D Program of China(No.2012BAC01B06)

摘　　要：A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0-8.0, at 28-30 ℃ and in the presence of NaCI concentrations from 0.0% to 0.5%. 16S rRNA gene sequence analysis indicated that strain 25-1m belongs to the genus Chryseobacterium within the family Flavo- bacteriaceae and is related most closely to C. camis G81m （96.4% similarity）, C. lathyri RBA2-6T （95.8% similarity）, and C. zeae JM1085T （95.8% similarity）. Its genomic DNA G＋C molar composition was 36.2%. The major cellular fatty acids were iso-C15：0 （44.0%）, iso-C17：0 3OH （19.8%） and C16：1 ω7c/16：1 ω6c （12.7%）. The only isoprenoid quinone was menaquinone 6 （MK-6）. The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1m and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T （ccmoc AB2015133T=DSM 100396T）.目的：鉴定菌株25-1~T是否是金黄杆菌属的一个新种。创新点：首次从空气中分离到金黄杆菌属的新种。方法：革兰氏染色镜检;磷钨酸染色然后透射电镜观察菌株25-1~T形态结构;全自动生理生化鉴定系统（Phoenix？-100）与传统生理生化反应管相结合;H890气象色谱仪进行脂肪酸组分分析;薄板双相层析进行极性脂组分分析;反相高压液相色谱分析法进行呼吸琨组分分析;熔解温度法检测G＋C摩尔含量;16SrRNA序列测定及系统发生分析。结论：根据传统特征分类研究结果（形态特征、培养特性和生理生化特征）、化学分类研究结果（脂肪酸组分、极性脂组分和呼吸琨组分）和遗传特征分类研究结果（G＋C摩尔含量、DNA同源性测定和16SrRNA序列测定及系统发生分析）得出菌株25-1~T为金黄杆菌属的一个新种。

关 键 词：Chryseobacterium chengduensis Giant panda 16S rRNA sequencing Phylogenetic analysis Strain 25-1T 

分 类 号：S852.61[农业科学—基础兽医学]