阿拉山口地区凶小库蚊核糖体DNA第二转录间隔区基因多态性的分析  

Genetic polymorphism analysis on rNDA-ITS2 of Culex modestus in Alataw Pass area

作　　者：尹小平 王安东[1,2] 罗丹[3] 田延河 梁臻 巴特 张江国 

机构地区：[1]新疆阿拉山口检验检疫局,新疆阿拉山口833418 [2]石河子大学动物科技学院,新疆石河子832003 [3]石河子大学医学院,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2016年第3期281-285,共5页Journal of Shihezi University(Natural Science)

基　　金：国家质检总局科技计划项目(2009CK211);新疆维吾尔自治区自然科学基金项目(201442137-1)

摘　　要：为了探讨中哈边境阿拉山口口岸地区凶小库蚊核糖体DNA第二转录间隔区(r DNA-ITS2)基因序列特征,分析其遗传变异和亲缘关系,为口岸地区蚊的鉴定及疾病防治奠定基础。本研究提取中哈边境阿拉山口口岸地区凶小库蚊蚊虫DNA,PCR扩增凶小库蚊的r DNA-ITS2基因并测序。将测序结果进行Blast分析,使用Mage 6.0构建分子进化树。结果显示:PCR扩增获得凶小库蚊第二转录间隔区(r DNA-ITS2)323 bp左右的基因片段。Blast显示与Culex restuans(U22136)具有较高的同源性,与其他库蚊属蚊种序列间差异集中在24-323 bp,差异表现为插入/缺失、转换/颠换、简单重复单元拷贝数不同等。结论:本研究报道了凶小库蚊第二转录间隔区(r DNA-ITS2)基因序。阿拉山口口岸凶小库蚊第二转录间隔区(r DNA-ITS2)基因区显示存在遗传多态性,可能与蚊虫地理区域,生态环境和贸易往来有一定关系。To explore sequence characteristics of r NDA- ITS2 in Culex modestus at Alataw Pass, China- Kazakhstan and to analyze the genetic variation of Culex modestus for providing the reference for the Culex classification research and scientific prevention and control of mosquito- borne diseases. Genomic DNA was extracted from Culex modestus at different sites, and the r NDA- ITS2 gene of Culex modestus were amplified by PCR. The sequence analysis by BLAST and phylogenetic analysis by Mage 6.0. We obtained the r NDA- ITS2 sequences were approximate 323 bp by PCR. Blast showed that the Culex modestus was high homology with Culex restuans（U22136）. There were transition,transversion and deletion in themono nucleotide of r NDA- ITS2 gene in the 24- 323 bp. r NDA- ITS2 gene sequence of Culex modestus were reported in this study. The r NDA- ITS2 gene of Culex modestus is genetic polymorphisms and they are probably associated with the ecological environment, geographical area and trade.

关 键 词：凶小库蚊 核糖体 RDNA-ITS2 PCR 阿拉山口地区 

分 类 号：S852.71[农业科学—基础兽医学] Q969.442[农业科学—兽医学]