思茅松优良无性系的聚类分析及精英无性系特异标记  

Genetic Analysis of Superior Clones and Specific Marker of Elite Clones of Pinus kesiya var. langbianensis

作　　者：朱云凤[1] 陈少瑜[1,2,3] 郝佳波[1,2,3] 吴涛[1,2,3] 

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省森林植物培育与开发利用重点实验室 [3]国家林业局开放性重点实验室云南珍稀濒特森林植物保护和繁育实验室,云南昆明650201

出　　处：《西部林业科学》2016年第4期141-146,共6页Journal of West China Forestry Science

基　　金：国家发改委生物育种高技术产业化专项“思茅松用材林良种高技术产业化示范与研究”项目资助的重点实验室开放项目“思茅松用材林优良无性系分子遗传分析”

摘　　要：以85个思茅松优良无性系为研究对象,通过RAPD分子标记方法对85个无性系的遗传距离进行分析,构建UPGMA聚类树状图,探讨85个无性系之间的遗传关系,为杂交亲本的选配和育种群体的多样性评价提供科学依据;此外,针对85个无性系中的33个精英无性系进行分析,探寻其特异标记,旨在为精英无性系的分子鉴别提供依据。研究结果表明,85个无性系可以划分为遗传差异较明显的3大类群,同时发现2个能够区分部分精英无性系的特异标记。By collecting 85 Pinus kesiya var. langbianensis clones as materials, and using RAPD markers as meth-od, the genetic distance was analyzed, and UPGMA were clustered in order to discover their genetic relationship and provide theoretical basis for diversity evaluation of hybrids and the breeding populations; The specific markers of 33 elite clones were also revealed for providing basis for molecular identification of elite clones. The results showed the 85 clones could be divided into 3 groups and 2 specific markers could be used to differentiate some of the elite clones.

关 键 词：思茅松 精英无性系 遗传多样性 特异标记 RAPD分子标记法 UPGMA聚类树状图 

分 类 号：S791.259[农业科学—林木遗传育种]