茅苍术麸炒前后胃黏膜保护作用的变化研究  

Study on Variation of Gastroprotective Effect of Atractylodes lancea Rhizome Before and After Processing

作　　者：于艳[1] 才谦[2] 贾天柱[2] 

机构地区：[1]辽宁中医药大学附属医院中医药实验中心,辽宁沈阳110034 [2]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中药材》2016年第4期760-763,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81202919);国家中医药管理局中医药行业科研专项(20110700712)

摘　　要：目的:比较茅苍术生品和麸炒品对胃溃疡大鼠的胃黏膜保护作用。方法:以乙酸诱导大鼠胃溃疡模型。采用酶联免疫吸附(ELISA)法检测血清及胃组织中表皮生长因子(EGF)和三叶因子2(TFF2)的含量。实时荧光定量聚合酶链式反应(RT-PCR)法检测胃组织中EGF和TFF2 mRNA的表达。免疫组织化学法检测胃组织中EGF和TFF2的蛋白表达。结果:茅苍术能够明显升高血清及胃组织中EGF和TFF2的含量,上调胃组织中EGF和TFF2mRNA的表达和二者的蛋白表达量,麸炒品的作用优于生品。结论:麸炒能增强茅苍术对乙酸致胃溃疡大鼠胃黏膜的保护作用。Objective： To compare the gastroprotective effect between the crude and bran-processed Atractylodes lancea rhizome in the rat model of gastric ulcer. Methods： The model of gastric ulcer was imitated by acetic acid in rats. The levels of epidermal growth factor（ EGF） and trefoil factor 2（ TFF2） in serum and stomach were determined by the double-antibody sandwich enzyme-linked immunosorbent assay（ ELISA）,and the mRNA expressions of EGF and TFF2 in stomach were analyzed by quantitative real-time reverse transcription polymerase chain reaction（ RT-PCR）. The protein expressions of EGF and TFF2 in stomach were examined by immunohistochemistry. Results： The productions of EGF,TFF2 and the mRNA expressions of EGF and TFF2 were notably up-regulated by both crude Atractylodes lancea rhizome and bran-processed Atractylodes lancea rhizome. Compared with the crude Atractylodes lancea rhizome,the processed Atractylodes lancea rhizome was more effective. Conclusion： The gastroprotective effect of Atractylodes lancea rhizome was enhanced after stir-frying with wheat bran in the rat model of gastric ulcer induced by acetic acid.

关 键 词：茅苍术 胃溃疡 炮制 麸炒 ELISA RT-PCR 免疫组织化学 

分 类 号：R283.1[医药卫生—中药学] R285.5[医药卫生—中医学]