猫爪草化学成分及抗耐药结核活性研究  

Constituents from Ranunculus ternatus and Their Inhibitory Effects on Multidrug-Resistant Tuberculosis

作　　者：熊英[1] 陈虹[1] 邓敏芝[1] 周斌[2] 邓可众[1] 罗永明[1] 

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]江西科技师范大学药学院,江西南昌330038

出　　处：《中药材》2016年第4期775-777,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(81060334)

摘　　要：目的：研究猫爪草的化学成分及其体外抗耐药结核活性。方法：采用ODS、Sephadex LH-20葡聚糖凝胶等多种柱色谱进行分离,利用NMR等波谱技术鉴定猫爪草的化学成分,利用绝对浓度法对分离得到的化合物进行抗耐药结核活性评价。结果：从猫爪草的抗耐药结核活性部位中分离得到7个化合物,分别鉴定为2-脱氧-D-核糖酸-1,4-内酯（1）、异麦芽酚-α-D-葡萄糖苷（2）、香草酸4-O-β-D-葡萄糖苷（3）、水杨苷（4）、正丁基-β-D-呋喃果糖苷（5）、3,4-二甲氧基苯甲酸（6）、咖啡酸（7）。结论：其中,化合物1~6为首次从毛茛属植物中分离得到。药敏试验表明,化合物1~4、6、7具有抗耐药结核活性。Objective： To explore the chemical constituents of Ranunculus ternatus and their inhibitory effects on multidrug-resistant tuberculosis. Methods： All compounds were separated by silica gel,Sephadex LH-20 column chromatography etc. Their structures were identified by NMR spectroscopy. The absolute concentration method was used for susceptibility test on multidrug-resistant tuberculosis strains with Ranunculus ternatus. Results： Seven compounds were isolated and identified as 2-deoxy-D-ribono-1,4-lactone（ 1）,isomaltol-α-D-glucoside（ 2）,vanillic acid-4-O-β-D-glucopyranoside（ 3）,salicoside（ 4）,n-butyl-β-D-fructofuranoside（ 5）,3,4-dimethoxybenzoic acid（ 6）,and caffeic acid（ 7）. Conclusion： Compounds 1 ~ 6 are isolated from this genus for the first time. All compounds except 5 of Ranunculus ternatus reveal modest activity to inhibit multidrug-resistant tuberculosis.

关 键 词：猫爪草 化学成分 耐药性结核 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]