半枝莲与白花蛇舌草及其药对石油醚部位成分分析及其抗子宫内膜癌细胞活性研究  

The Component Analysis of Petroleum Ether Extract in Scutellariae barbatae,Hedyotis diffusa and the Herb Pair and the Investigation of Anti-Endometrial Cancer Cells Activity

作　　者：逄亚楠 国锦 闫辉[1] 容蓉[1] 巩丽丽[1] 蒋海强[1] 吕青涛[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《中药材》2016年第4期789-794,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81173614)

摘　　要：目的:对半枝莲、白花蛇舌草及其药对石油醚部位成分进行分析,并研究体外抗子宫内膜癌细胞活性。方法:采用GC-MS结合保留指数(Kovats retention index,KI)对主要成分进行分析和鉴定;甲基偶氮唑盐类(MTT)法研究三者的抗子宫内膜癌细胞活性。结果:通过GC-MS分析鉴定出半枝莲、白花蛇舌草及药对石油醚部位中同时含有不饱和脂肪酸、酯类、甾醇类等成分,白花蛇舌草和药对相区别于半枝莲的石油醚部位又含有较多蒽醌类化合物。半枝莲、白花蛇舌草及其药对石油醚部位抗子宫内膜癌细胞HEC-1A的IC50值分别为275.204μg/m L,105.826μg/m L,148.645μg/m L,抗子宫内膜癌细胞Ishikawa的IC50值分别为189.114μg/m L,77.974μg/m L,137.999μg/m L。结论:半枝莲与白花蛇舌草及其药对石油醚部位对子宫内膜癌细胞HEC-1A和Ishikawa均有抑制作用,其中白花蛇舌草的抗子宫内膜癌细胞活性最强,推测可能与含量较高的蒽醌类化合物有关。Objective： To investigate the chemical components and the activity of anti-endometrial cancer cells of the petroleum ether extract in Scutellariae barbatae and Hedyotis diffusa and the herb pair. Methods： Main composition analysis and identification were determined by the GC-MS technology combined with Kovats retention index（ KI）. Activity of anti-endometrial cancer cells was researched by MTT assay. Results： Unsaturated fatty acid,esters,sterol and other compounds in Scutellariae barbatae,Hedyotis diffusa and the herb pair were identified by GC-MS. Hedyotis diffusa and the herb pair contained more anthraquinones which distinguished from Scutellariae barbatae. The IC50 values for HEC-1A cells of petroleum ether extract in Scutellariae barbatae and Hedyotis diffusa and the herb pair were 275. 204 μg / m L,105. 826 μg / m L,148. 645 μg / m L. The IC50 values for Ishikawa cells of petroleum ether extract in Scutellariae barbatae and Hedyotis diffusa and the herb pair are 189. 114 μg / m L,77. 974 μg / m L,137. 999 μg / m L. Conclusion： Petroleum ether extract in Scutellariac barbatae and Hedyotis diffusa and the herb pair has inhibition effect on the proliferation of HEC-1A and Ishikawa cells,the Hedyotis diffusa has the strongest activity of anti-endometrial cancer. It is speculated that the strongest activity could be related to the higher content of anthraquinones.

关 键 词：半枝莲 白花蛇舌草 药对 石油醚部位 GC-MS 子宫内膜癌细胞 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]