大黄对慢性便秘模型大鼠结肠血管活性肠肽水平及肠动力影响研究  

Effect of rhubarb on the vasoactive intestinal peptide and motility in colon tissues of rats with chronic constipation

作　　者：杜丽娟[1,2] 占煜 吴至久[1] 孔鹏飞[1] 赵兵[1] 陈泰宇[1] 唐学贵[1] 

机构地区：[1]川北医学院附属医院肛肠科,四川南充637000 [2]成都中医药大学,四川成都610072 [3]成都市第一人民医院,四川成都610071

出　　处：《川北医学院学报》2016年第4期470-472,共3页Journal of North Sichuan Medical College

基　　金：国家自然科学基金项目(81273663);四川省教育厅青年基金项目(11ZB184);川北医学院科研发展计划项目(CBY12-A-ZP02)

摘　　要：目的:采用洛哌丁胺灌胃建立便秘大鼠模型,观察大黄对便秘模型大鼠血管活性肠肽(vasoactive intestinal peptide,VIP)水平的影响。方法:健康Wistar大鼠18只,随机分为3组,空白组、模型组、大黄组。模型组及大黄组采用配制好的含洛哌丁胺(1.5 mg·kg^(-1)·d^(-1))混悬液2 m L灌胃7 d。模型建立后,大黄组以大黄(5 mg/kg)混悬液2 m L灌胃。检测结肠传输,ELISA检测AQP3含量。结果:(1)造模后大鼠的体重增加、结肠存留粪便增多、粪便含水量降低、肠道传输时间延长(P<0.05);(2)大黄干预组大鼠大便量、粪便含水量增加(P<0.05);(3)大黄干预便秘大鼠,其结肠传输时间缩短,VIP水平明显低于模型组(P<0.05)。结论:大黄可加快肠道传输,可能与降低大鼠结肠中VIP水平,促进肠蠕动有关。Objectlve：To observe the effect of Rhubarb on the vasoactive intestinal peptide （VIP） in colon tissues in the Lop- ersmide-induced constipated rats. Methods：18 healthy Wistar rats were randomly divided into 3 groups,the control group,model group and rhubarb group. Model group and rhubarb group were prepared with the fittest dosage of Lopersmide（ 1.5 mg· kg 1 · d^-1 ） mixed suspension 2 mL lavage 7 d. After model establishment,rhubarb group with rhubarb （5 mg/kg） mixed suspension 2 mL Iavage. Detection of colonic transmission,ELISA detection of AQP3 content. Results ： （ 1 ） After building the model, the rats＇ weight gain, the colon feces increase, waste water content reduce and the transmission time of the intestinal tract prolonged （ P 〈 0. 05 ） ;2 ） The feces and the waste water content of rats in rhubarb group were increased obviously （ P 〈 0.05 ）. （ 3 ） Rhubarb intervention in rats with constipation, the colonic transit time was shortened,VIP level was significantly lower than the model group （ P 〈 0.05 ）. Conclusion： Rhubarb can aecele intestinal transmission,may be related to reducing the level of VIP in the colon of rats,and promoting the intestinal peristal- sis.

关 键 词：大黄 血管活性肠肽 便秘 

分 类 号：R256.25[医药卫生—中医内科学]