人参PgPI基因3'端序列的克隆与分析  

Cloning and Sequence Analysis of PgPI Gene from Panax ginseng C.A.Mey

作　　者：齐伟辰[1] 陆静梅[2] 杨利民[3] 刘翠晶[3] 

机构地区：[1]长春中医药大学药学院,长春130117 [2]东北师范大学生命科学学院,长春130024 [3]吉林农业大学中药材学院,长春130118

出　　处：《吉林农业大学学报》2016年第4期426-430,共5页Journal of Jilin Agricultural University

基　　金：国家自然科学基金青年科学基金项目(31200178);国家自然科学基金项目(41271231;31270371);吉林省科技发展计划项目(20120270)

摘　　要：MADS-box基因编码的蛋白为转录因子,在被子植物花发育调控中发挥关键作用。其中,PI-like基因主要调控花瓣和雄蕊的形成。利用3'-c DNA末端快速扩增技术,从人参花蕾中克隆得到1个PI-like基因,命名为Pg PI。该基因的3'端序列长558 bp,编码186个氨基酸,GC含量为41.9%。序列比对结果显示,Pg PI与Hh PI(Hedera helix,ADM88561)的相似度为87%。聚类分析结果表明,人参与洋常春藤的PI-like基因聚为一支,进化距离较其他物种近。As transcriptional regulation factors, MADS-Box proteins play key roles in flower development of angiosperms. The PI-like genes control the identity of petals and stamens. A homologue of PI, designated as PgPI, was isolated from cultivar ginseng flower buds by rapid amplification of cD- NA ends （RACE）. The partial cDNA sequence of PgPI gene was 558 bp in length, which encoded a protein of 186 amino acids. The GC content was 41.9%. The alignment showed that PgPI had a high similarity with HhPI （87%）（Hedera helix, ADM88561 ）. The clustering analysis showed that PgPI and HhPI were in the same branch.

关 键 词：人参 PgPI基因 序列分析 克隆 

分 类 号：Q785[生物学—分子生物学] S567.51[农业科学—中草药栽培]