草原红牛和中国荷斯坦牛肌肉组织SSH文库的构建及初步分析  

Construction and Preliminary Analysis of Muscle SSH c DNA Library of Red Steppes and Chinese Holstein Cattle

作　　者：王晓楠[1] 东雪 徐静[1] 单雪松[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《吉林农业大学学报》2016年第4期452-455,472,共5页Journal of Jilin Agricultural University

基　　金：国家"863"计划项目(2008AA101010)

摘　　要：利用抑制性消减杂交(Suppression subtractive hybridization,SSH)方法构建了中国草原红牛与中国荷斯坦牛背最长肌差异表达消减c DNA文库,共获得859个差异表达克隆,对全文库进行了测序并与Gen Bank数据库进行比对分析。结果表明:共获得789个新ESTs,84个未知功能蛋白基因,356个已知功能蛋白基因。在已知功能蛋白基因中,表达上调的基因有123个,表达下调的基因有233个。差异表达基因可能与肉质性状有关,可以作为肉质性状的候选基因进行深入研究。Suppression subtractive hybridization （SSH） was used to construct cDNA library of the Chinese Red Steppes and Chinese Holstein Cattle dorsi muscle. Eight hundred and fifty-nine differentially expressed clones obtained from the library were sequenced and analyzed with blasting program of GenBank for sequence comparison. The results showed that a total of 789 new ESTs, 84 unknown genes and 356 functional genes were obtained in this library. Among these functional protein genes, there are 123 up-regulated genes and 233 down-regulated genes in Red Steppes cattle compared with Chinese Holstein cattle. These differentially expressed genes may be related to meat quality traits and can be used as candidate genes for meat quality traits.

关 键 词：抑制性消减杂交 中国草原红牛 中国荷斯坦牛 

分 类 号：S823.2[农业科学—畜牧学] Q75[农业科学—畜牧兽医]