HPLC法测定愈伤灵胶囊中尿囊素与羟基红花黄色素A的含量  

HPLC determination of allantoin and hydroxysafflor yellow A in Yushangling capsules

作　　者：易小兰 

机构地区：[1]湖南汉森医药研究有限公司,长沙410013

出　　处：《中南药学》2016年第7期759-762,共4页Central South Pharmacy

基　　金：湖南省科技厅战略新兴产业项目(No.2014GK1046)

摘　　要：目的分别建立一种测定愈伤灵胶囊中尿囊素与羟基红花黄色素A含量的方法。方法采用高效液相色谱法,色谱柱为Thermo Syncronis HILIC柱（4.6 mm×250 mm,5μm）。以乙腈-水（98∶2）为流动相,191 nm为检测波长测定尿囊素含量;以乙腈-0.7%磷酸（15∶85）为流动相,337 nm为检测波长测定羟基红花黄色素A含量。结果尿囊素在37.62~1254 ng内与峰面积线性关系良好,r为0.9999,平均回收率为99.0%;羟基红花黄色素A在48.14~722.06 ng内与峰面积线性关系良好,r为0.9997,平均回收率为95.8%。结论本方法专属性强、重复性好,能有效地控制愈伤灵胶囊中土鳖虫与红花的质量,对愈伤灵胶囊质量标准的提高有一定参考价值。Objective To establish an HPLC method for the determination of allantoin and hydroxysafflor yellow A in Yushangling capsules. Methods Thermo Syncronis HILIC column（250 mm×4.6 mm, 5 μm） was used. The mobile phase was acetonitrile-water（98∶2） and the detection wavelength was 191 nm for allantoin. The mobile phase of acetonitrile-0.7%phosphate（15∶85） and the detection wavelength was 337 nm for hydroxysafflor yellow A. Results Allantoin was linear within 37.62- 1254 ng（r = 0.9999）, and the average recovery was 99.0%; Hydroxysafflor yellow A was linear within 48.14- 722.06 ng（r = 0.9997）, and the average recovery was 95.8%. Conclusion The method is accurate with good reproducibility, which can control the quality of eupolyphaga and carihami flos in Yushangling capsules effectively, and is valuable to improve the quality standard of Yushangling capsules.

关 键 词：愈伤灵胶囊 土鳖虫 尿囊素 红花 羟基红花黄色素A 

分 类 号：R917[医药卫生—药物分析学]