三黄鸡群混合感染禽白血病病毒和马立克氏病病毒导致的临床肿瘤初步研究  

Preliminary study of the Sanhuang chicken flock experienced clinic tumors caused by the mixed infection of avian leukosis virus(ALV) and Marek's disease virus(MDV)

作　　者：林璐璐[1] 杨永立[1] 王培坤[1] 邹广珍[1] 韦平[1] 

机构地区：[1]广西大学养禽与禽病学研究所,南宁530004

出　　处：《黑龙江畜牧兽医》2016年第8期154-157,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家公益性(农业)行业科研专项(201203055);国家现代农业产业技术体系广西肉鸡产业创新团队建设项目(nycytxgxcxtd-04-20-2)

摘　　要：为了进一步研究禽白血病病毒（avianleukosisvirus，ALV）与马立克氏病病毒（Marek’s dis—easevirus，MDV）的流行趋势和致病机理，试验对三黄鸡疑似肿瘤样品中ALV—J的gp85基因和MDV的meq基因进行PCR扩增、克隆和序列测定与比较分析。结果表明：GX15PP03-ALV的gp85基因与ALV—J英国原型株HPRS-103的核苷酸及氨基酸的同源性分别为97．9％和96．8％，与其他8株国内外参考株的核苷酸及氨基酸的同源性分别为87．9％-99．7％和83．7％～99．0％；GX15PP03-MDV的meq基因与8个MDV强毒参考株的核苷酸及氨基酸的同源性分别为99．0％-99．8％和97．4％～99．4％，而且具有强毒株的序列特征。说明ALV—J和MDV混合感染是导致该鸡群临床爆发肿瘤的主要原因。To further study the popular trend and pathogenesis of avian leukosis virus （ALV） and Marek＇s disease virus （MDV）, the gp85 gene of ALV - J and the meq gene of MDV in the suspected tumor samples from Sanhuang chickens were used for PCR amplification, cloning, se- quencing and comparative analysis. The results showed that the gp85 gene of GX15PP03 - ALV exhibited 97.9% nucleotide homology and 96. 8% amino - acid homology with the British prototype ALV - J strain HPRS - 103, and shared 87.9% to 99.7% nucleotide homology and 83. 7% to 99.0% amino - acid homology with those of the eight reference strains at home and abroad. The meq gene of GX15PP03 - MDV shared 99.0% to 99.8% nucleotide homology and 97.4% to 99.4% amino - acid homology with those of the eight highly virulent reference strains, and possessed the sequence characteristics of the highly virulent strains. The results indicate that the mixed infection of ALV - J and MDV may be the main cause of the outbreak of clinical tumors in the flock of chickens.

关 键 词：J亚群禽白血病病毒 GP85基因 马立克氏病病毒 MEQ基因 混合感染 序列分析 

分 类 号：S853.53[农业科学—临床兽医学] S831[农业科学—兽医学]